Literature DB >> 6188965

Immunoblot identification of phosphorylated basic proteins of rat and rabbit CNS and PNS myelin: evidence for four phosphorylated basic proteins and P2 in rat PNS myelin.

W R Gilbert, M M Garwood, D Agrawal, R E Schmidt, H C Agrawal.   

Abstract

The immunoblot technique permits the visualization of proteins following their separation on acrylamide gels, transfer to cellulose nitrate sheets and subsequent staining with antiserum. We have utilized this technique to demonstrate the presence of four basic proteins in rat PNS myelin with molecular weights of 21K, 18K, 17K, and 14K. Similarly, we demonstrated the presence of two basic proteins in rabbit PNS myelin (molecular weights of 21K and 18K). Exposure of the immunostained cellulose nitrate strips to X-ray film revealed the phosphorylation of four and two basic proteins in rat and rabbit PNS myelin, respectively. These basic proteins were present in the CNS myelin of the two species and were also phosphorylated. Because of the sensitivity of the immunoblot technique, it was also possible for us to visualize the P2 protein in both rat and rabbit PNS myelin.

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Year:  1982        PMID： 6188965     DOI： 10.1007/bf00965092

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  21 in total

1.  Protein kinases associated with peripheral nerve myelin. 1. Phosphorylation of endogenous myelin proteins and exogenous substrates.

Authors:  H Singh; N Spritz
Journal:  Biochim Biophys Acta       Date:  1976-10-05

2.  Effect of N,N,N',N'-tetramethylethylenediamine on the migration of proteins in SDS polyacrylamide gels.

Authors:  J H Allison; H C Agrawal; B W Moore
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

3.  Partial characterization of a new myelin protein component.

Authors:  H C Agrawal; R M Burton; M A Fishman; R F Mitchell; A L Prensky
Journal:  J Neurochem       Date:  1972-09       Impact factor: 5.372

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Characterization of the basic proteins from rodent peripheral nervous system myelin.

Authors:  S Greenfield; S W Brostoff; E L Hogan
Journal:  J Neurochem       Date:  1980-02       Impact factor: 5.372

6.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

7.  Purification and immunohistochemical localization of rat brain myelin proteolipid protein.

Authors:  H C Agrawal; B K Hartman; W T Shearer; S Kalmbach; F L Margolis
Journal:  J Neurochem       Date:  1977-03       Impact factor: 5.372

8.  Phosphorylation and fucosylation of myelin protein in vitro by sciatic nerve from developing rats.

Authors:  R C Wiggins; P Morell
Journal:  J Neurochem       Date:  1980-03       Impact factor: 5.372

9.  Isolation and characterization of a protein from sciatic nerve myelin responsible for experimental allergic neuritis.

Authors:  S Brostoff; P Burnett; P Lampert; E H Eylar
Journal:  Nat New Biol       Date:  1972-02-16

10.  A comparative study of the immunohistochemical localization of basic protein to myelin and oligodendrocytes in rat and chicken brain.

Authors:  B K Hartman; H C Agrawal; S Kalmbach; W T Shearer
Journal:  J Comp Neurol       Date:  1979-11-15       Impact factor: 3.215
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  11 in total

1.  Immunochemical evidence of phosphorylation of a new 23K basic protein in rat brain myelin.

Authors:  H C Agrawal; D Agrawal; R P Jenkins
Journal:  Neurochem Res       Date:  1986-03       Impact factor: 3.996

Review 2.  Phosphorylation of myelin protein: recent advances.

Authors:  J Eichberg; S Iyer
Journal:  Neurochem Res       Date:  1996-04       Impact factor: 3.996

3.  Proteolipid protein and DM-20 are synthesized by Schwann cells, present in myelin membrane, but they are not fatty acylated.

Authors:  H C Agrawal; D Agrawal
Journal:  Neurochem Res       Date:  1991-08       Impact factor: 3.996

4.  In vitro acylation of myelin PLP and DM-20 in the quaking mouse brain.

Authors:  H C Agrawal; D Agrawal; T Yoshimura; J A Benjamins
Journal:  Neurochem Res       Date:  1987-09       Impact factor: 3.996

5.  Tumor promoters accentuate phosphorylation of PO: evidence for the presence of protein kinase C in purified PNS myelin.

Authors:  H C Agrawal; D Agrawal
Journal:  Neurochem Res       Date:  1989-05       Impact factor: 3.996

6.  Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting.

Authors:  N Kerlero De Rosbo; P R Carnegie; C C Bernard; D S Linthicum
Journal:  Neurochem Res       Date:  1984-10       Impact factor: 3.996

7.  In vivo acylation of proteolipid protein and DM-20 in myelin and myelin subfractions of developing rat brain: immunoblot identification of acylated PLP and DM-20.

Authors:  M M Garwood; W R Gilbert; H C Agrawal
Journal:  Neurochem Res       Date:  1983-05       Impact factor: 3.996

8.  P0 and myelin basic protein-like immunoreactivities following ligation of the sciatic nerve in the rat.

Authors:  C Patricia Setton-Avruj; Jorge B Aquino; Carolina J Goedelman; Eduardo F Soto; Marcelo J Villar
Journal:  Neurochem Res       Date:  2002-11       Impact factor: 3.996

9.  Myelin basic protein and P2 protein are not immunohistochemical markers for Schwann cell neoplasms. A comparative study using antisera to S-100, P2, and myelin basic proteins.

Authors:  H B Clark; J J Minesky; D Agrawal; H C Agrawal
Journal:  Am J Pathol       Date:  1985-10       Impact factor: 4.307

10.  Immunoblot identification of glial fibrillary acidic protein in rat sciatic nerve, brain, and spinal cord during development.

Authors:  M J Noetzel; H C Agrawal
Journal:  Neurochem Res       Date:  1985-06       Impact factor: 3.996
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