Literature DB >> 6188723

Technical difficulties overcome in the use of Lowicryl 4KM EM embedding resin.

P R Fryer, C Wells, A Ratcliffe.   

Abstract

Several technical difficulties have been overcome in the use of Lowicryl 4KM resin. In order to embed and section tissue satisfactorily in the resin, it has been found necessary to thoroughly degass the resin before infiltration and polymerisation. After irradiation with UV light, the blocks are further polymerised by exposure to daylight for 2-3 weeks and then stored under partial vacuum over dessicant.

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Year:  1983        PMID： 6188723     DOI： 10.1007/bf00496645

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  3 in total

1.  Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.

Authors:  J Roth; M Bendayan; E Carlemalm; W Villiger; M Garavito
Journal:  J Histochem Cytochem       Date:  1981-05       Impact factor: 2.479

2.  Immunocytochemical localization of kallikrein in the rat exocrine pancreas.

Authors:  M Bendayan; T B Orstavik
Journal:  J Histochem Cytochem       Date:  1982-01       Impact factor: 2.479

3.  Immunocytochemical localization of mitochondrial proteins in the rat hepatocyte.

Authors:  M Bendayan; G C Shore
Journal:  J Histochem Cytochem       Date:  1982-02       Impact factor: 2.479
  3 in total
  9 in total

1.  Intracellular localization of rotaviral proteins.

Authors:  S C Richardson; L E Mercer; S Sonza; I H Holmes
Journal:  Arch Virol       Date:  1986       Impact factor: 2.574

2.  Immuno-electron microscopy of chondrocyte-derived cells in the rheumatoid cartilage-pannus junction.

Authors:  K D Muirden; S A Allard; K Rogers; R N Maini
Journal:  Rheumatol Int       Date:  1988       Impact factor: 2.631

3.  Immuno-electron-microscopic analysis of the basal route of secretion in the subcommissural organ of the rabbit.

Authors:  W Lösecke; W Naumann; G Sterba
Journal:  Cell Tissue Res       Date:  1986       Impact factor: 5.249

4.  Preparation and discharge of secretion in the subcommissural organ of the rat. An electron-microscopic immunocytochemical study.

Authors:  W Lösecke; W Naumann; G Sterba
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

5.  Comparative investigations of the morphology and chemical composition of the eggshells of Acanthocephala. I. Macracanthorhynchus hirudinaceus (Archiacanthocephala).

Authors:  W Peters; H Taraschewski; I Latka
Journal:  Parasitol Res       Date:  1991       Impact factor: 2.289

6.  A type VI collagen-related glycopolypeptide is the major concanavalin A-binding component in pig skin.

Authors:  I A King; A Tabiowo; P R Fryer; F M Pope
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

7.  Distribution and phosphorylation of the basic protein P6.9 of Autographa californica nucleopolyhedrovirus.

Authors:  XiaoXiao Liu; Haizhou Zhao; Zhixin Fang; Meijin Yuan; Kai Yang; Yi Pang
Journal:  J Virol       Date:  2012-09-05       Impact factor: 5.103

8.  Evidence that major 78-44-kD concanavalin A-binding glycopolypeptides in pig epidermis arise from the degradation of desmosomal glycoproteins during terminal differentiation.

Authors:  I A King; A Tabiowo; P R Fryer
Journal:  J Cell Biol       Date:  1987-12       Impact factor: 10.539

9.  Proteoglycan biosynthesis in chondrocytes: protein A-gold localization of proteoglycan protein core and chondroitin sulfate within Golgi subcompartments.

Authors:  A Ratcliffe; P R Fryer; T E Hardingham
Journal:  J Cell Biol       Date:  1985-12       Impact factor: 10.539
  9 in total

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