Literature DB >> 6187008

Antibodies specific for NADPH-binding region of enzymes possessing dehydrogenase activities.

S S Katiyar, J W Porter.   

Abstract

The results reported in this paper show the presence of a population of antibodies in rabbit polyclonal antiserum that recognize an antigenic site at the NADPH-binding region of enzymes possessing dehydrogenase activities. Antisera from rabbits immunized with glucose-6-phosphate dehydrogenase or fatty acid synthetase were found to inactivate the enzyme dihydrofolate reductase. The inhibitory effect of this site-specific antibody is a time- and concentration-dependent reaction. This immunoinactivation is prevented by preincubation of the enzyme with NADPH.

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Year:  1983        PMID： 6187008      PMCID： PMC393566          DOI： 10.1073/pnas.80.5.1221

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Glucose-6-phosphate dehydrogenase. Purification and partial characterization.

Authors:  M I Kanji; M L Toews; W R Carper
Journal:  J Biol Chem       Date:  1976-04-25       Impact factor: 5.157

2.  Fatty acid synthase from pigeon liver.

Authors:  R A Muesing; J W Porter
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

3.  Fatty acid synthetase. A steady state kinetic analysis of the reaction catalyzed by the enzyme from pigeon liver.

Authors:  S S Katiyar; W W Cleland; J W Porter
Journal:  J Biol Chem       Date:  1975-04-10       Impact factor: 5.157

4.  Chemical and biological evolution of nucleotide-binding protein.

Authors:  M G Rossmann; D Moras; K W Olsen
Journal:  Nature       Date:  1974-07-19       Impact factor: 49.962

5.  Similarity in the sequence of Escherichia coli dihydrofolate reductase with other pyridine nucleotide-requiring enzymes.

Authors:  C D Bennett
Journal:  Nature       Date:  1974-03-01       Impact factor: 49.962

6.  Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli.

Authors:  M Poe; N J Greenfield; J M Hirshfield; M N Williams; K Hoogsteen
Journal:  Biochemistry       Date:  1972-03-14       Impact factor: 3.162

7.  Immunologic comparison of pancreatic ribonucleases.

Authors:  G W Welling; G Groen; J J Beintema; M Emmens; F P Schröder
Journal:  Immunochemistry       Date:  1976-08

8.  Comparative immunochemical studies of primate hemoglobins.

Authors:  F A Garver; D W Talmage
Journal:  Biochem Genet       Date:  1975-12       Impact factor: 1.890

9.  Amino acid substitution and the antigenicity of globular proteins.

Authors:  M Reichlin
Journal:  Adv Immunol       Date:  1975       Impact factor: 3.543

10.  Production of antibodies that bind biotin and inhibit biotin containing enzymes.

Authors:  M Berger
Journal:  Biochemistry       Date:  1975-06-03       Impact factor: 3.162
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  5 in total

1.  Purification of nucleotide-requiring enzymes by immunoaffinity chromatography.

Authors:  S R Stapleton; J W Porter
Journal:  Biochem J       Date:  1985-03-15       Impact factor: 3.857

2.  Nitrate Reductase from Monoraphidium braunii: Immunocytochemical Localization and Immunological Characterization.

Authors:  A Lopez-Ruiz; J M Roldan; J P Verbelen; J Diez
Journal:  Plant Physiol       Date:  1985-07       Impact factor: 8.340

3.  Immunological study of lactate dehydrogenase from Streptococcus mutans and evidence of common antigenic domains with lactate dehydrogenases from lactic bacteria.

Authors:  P Sommer; J P Klein; J A Ogier; R M Frank
Journal:  Infect Immun       Date:  1986-01       Impact factor: 3.441

4.  Immunological crossreactivity of eukaryotic C1-tetrahydrofolate synthase and prokaryotic 10-formyltetrahydrofolate synthetase.

Authors:  C Staben; J C Rabinowitz
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

5.  Cross-reacting human and rabbit antibodies to antigens of Histoplasma capsulatum, Candida albicans, and Saccharomyces cerevisiae.

Authors:  B V Kumar; G Medoff; G S Kobayashi; W L Sieling
Journal:  Infect Immun       Date:  1985-06       Impact factor: 3.441
  5 in total

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