| Literature DB >> 6186901 |
A P Ryskov, M I Prosnyak, S A Korneev, S A Limborska.
Abstract
Partially purified human procollagen mRNAs have been copied using reverse transcriptase from avian myeloblastosis virus. Under standard conditions, using a high deoxynucleotide concentration, only incomplete cDNAs corresponding to one-fifth to one-half of the template could be synthesized. Addition of a ribonuclease inhibitor from human placenta in the reaction mixture allowed the synthesis of full-length cDNA copies from extremely long procollagen mRNA.Entities:
Mesh：
Substances：
Year: 1982 PMID： 6186901 DOI： 10.1007/bf00776582
Source DB: PubMed Journal: Mol Biol Rep ISSN： 0301-4851 Impact factor: 2.316