| Literature DB >> 6186744 |
H Lutz, N C Pedersen, R Durbin, G H Theilen.
Abstract
Three different monoclonal antibodies were developed against the major core protein (p27) of feline leukemia virus (FeLV). Each antibody was directed against a different epitope of the species-specific portion of FeLV-p27. The 3 antibodies reacted with 5 different isolates of FeLV but not with 7 other retroviruses (MuLV (Rauscher), MuLV (AKR), MPMV, MMTV, SMRV, BAEV, RD 114). These monoclonal antibodies could readily be adapted to an enzyme-linked immunosorbent assay (ELISA) for the specific measurement of FeLV-p27. When compared in an ELISA with conventional reagents, the battery of monoclonal antibodies proved to be as sensitive as conventional polyclonal antibodies.Entities:
Mesh：
Substances：
Year: 1983 PMID： 6186744 DOI： 10.1016/0022-1759(83)90413-1
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303