Literature DB >> 6186695

A modified tetrazolium reaction for identifying malignant cells from gastric and colonic cancer.

K S Ibrahim, O A Husain, L Bitensky, J Chayen.   

Abstract

When non-malignant cells were reacted for glucose-6-phosphate dehydrogenase activity, with neotetrazolium chloride as the indicator of the activity, oxygen competed with the neotetrazolium and nullified the reaction. In contrast, about 30% of the activity was retained in malignant cells, in sections and in smears, from cancer of the stomach or colon. This could provide the basis of a qualitative (black-or-white) functional test for distinguishing malignant cells in these conditions.

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Year:  1983        PMID： 6186695      PMCID： PMC498138          DOI： 10.1136/jcp.36.2.133

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  10 in total

1.  GLUCOSE-6-PHOSPHATE DEHYDROGENASE ACTIVITY IN HYPERPLASTIC AND NEOPLASTIC LESIONS OF THE BREAST. A HISTOCHEMICAL STUDY.

Authors:  R B COHEN
Journal:  Cancer       Date:  1964-08       Impact factor: 6.860

2.  6-PHOSPHOGLUCONATE DEHYDROGENASE ACTIVITY IN VAGINAL FLUID: LIMITATIONS AS A SCREENING TEST FOR GENITAL CANCER.

Authors:  C B CAMERON; O A HUSAIN
Journal:  Br Med J       Date:  1965-06-12

3.  Histochemical demonstration of 6-phosphogluconate dehydrogenase in proliferating and malignant cells.

Authors:  J CHAYEN; L BITENSKY; E K AVES; G R JONES; A A SILCOX; G J CUNNINGHAM
Journal:  Nature       Date:  1962-08-18       Impact factor: 49.962

4.  A new enzyme test for gynaecological cancer. 6-Phosphogluconate dehydrogenase activity in vaginal fluid.

Authors:  D G BONHAM; D F GIBBS
Journal:  Br Med J       Date:  1962-09-29

5.  Microdensitometry.

Authors:  L Bitensky
Journal:  Ciba Found Symp       Date:  1979

6.  A histochemical study of experimentally induced malignant skin tumours.

Authors:  J V Diengdoh; J Chayen
Journal:  Br J Dermatol       Date:  1969-11       Impact factor: 9.302

7.  Studies on the reduction of tetrazolium salts. II. The measurement of the half reduced and fully reduced formazans of neotetrazolium chloride in tissue sections.

Authors:  R G Butcher; F P Altman
Journal:  Histochemie       Date:  1973-12-31

8.  Cytologic studies of glucose-6-phosphate dehydrogenase in malignancy.

Authors:  H J Cohen; A Elizalde; S P Miller
Journal:  Cancer       Date:  1968-06       Impact factor: 6.860

9.  Vaginal fluid enzymology. A new assay method with enzyme-potassium ratios.

Authors:  D F Gibbs; A H Labrum; B H Stagg
Journal:  Am J Obstet Gynecol       Date:  1968-12-01       Impact factor: 8.661

Review 10.  Enzymology of cancer cells (first of two parts).

Authors:  G Weber
Journal:  N Engl J Med       Date:  1977-03-03       Impact factor: 91.245
  10 in total
  4 in total

1.  Antioxidant and glutathione-associated enzymes in Wilms' tumour after chemotherapy.

Authors:  J Gajewska; M Szczypka; T Izbicki; T Klepacka; T Laskowska-Klita
Journal:  J Cancer Res Clin Oncol       Date:  1996       Impact factor: 4.553

Review 2.  Cytochemical detection of cancer: a review.

Authors:  L Bitensky; J Chayen; O A Husain
Journal:  J R Soc Med       Date:  1984-08       Impact factor: 5.344

3.  Cytochemical investigation of glucose-6-phosphate dehydrogenase activity in rat mammary tissue.

Authors:  P P Smyth; E T Barron; I Tobbia; N J O'Higgins
Journal:  Br J Exp Pathol       Date:  1987-02

4.  Reaction rate studies of glucose-6-phosphate dehydrogenase activity in sections of rat liver using four tetrazolium salts.

Authors:  R G Butcher; C J Van Noorden
Journal:  Histochem J       Date:  1985-09
  4 in total

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