Antigenic cross-reactivities between mammalian hexokinases.

Immunoelectrophoresis and gel diffusion techniques have demonstrated that, contrary to previous reports, antigenic cross-reactivity does exist between the three major rat hexokinase isoenzymes. Immune inhibition and immune precipitation assays performed in parallel generally failed to detect this cross-reactivity and possible reasons for this failure are discussed. The inability of the latter assay systems to detect cross-reactivity would seem to account for the discrepancies between the present results and those obtained in earlier studies.