Literature DB >> 6185281

Visualization of Ag-NOR proteins on nucleolar transcriptional units in molecular spreads.

N Angelier, D Hernandez-Verdun, M Bouteille.   

Abstract

Application of NOR silver staining to Pleurodeles oocyte nuclei showed selective silver deposits on the fibrillar part of the nucleoli in situ. This staining was adapted to nucleoli spread on grids, by a procedure which allowed the spreading of transcription units from both nucleoli and lampbrush chromosomes on the same grids. This permitted localization of the predominant nucleolar Ag-stained proteins on the nucleolar transcriptional units and not on the lampbrush chromosomes. These proteins were found exclusively on the transcribed part of the nucleolar genes and were not seen in apparently untranscribed spacer regions. The proteins seemed preferentially located on the DNP axis rather than on the RNP fibrils.

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Year:  1982        PMID： 6185281     DOI： 10.1007/bf00285609

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  34 in total

1.  Electron microscopic autoradiography of isolated nucleolar transcription units.

Authors:  N Angelier; D Hemon; M Bouteille
Journal:  Exp Cell Res       Date:  1976-07       Impact factor: 3.905

2.  Silver staining of NORs in electron microscopy.

Authors:  C A Bourgeois; D Hernandez-Verdun; J Hubert; M Bouteille
Journal:  Exp Cell Res       Date:  1979-10-15       Impact factor: 3.905

3.  [Ultrastructural identification of the nucleolus organizer by the silver staining technic].

Authors:  D Hernandez-Verdun; J Hubert; C Bourgeois; M Bouteille
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1978-12

4.  Evidence from studies on segregated nucleoli that nucleolar silver staining proteins C23 and B23 are in the fibrillar component.

Authors:  Y Daskal; K Smetana; H Busch
Journal:  Exp Cell Res       Date:  1980-06       Impact factor: 3.905

5.  [On a method of staining nucleoli both for light- and electron-microscopy (author's transl)].

Authors:  A Ellinger; F Wachtler
Journal:  Mikroskopie       Date:  1980-12

6.  Peripheral RNA synthesis of fibrillar center in nucleoli of Japanese quail oocytes and somatic cells.

Authors:  C Mirre; A Stahl
Journal:  J Ultrastruct Res       Date:  1978-09

7.  Localization of silver staining in interphase, prophase and metaphase lymphocytes.

Authors:  F Pelliccia; A de Capoa; G Belloni; A Rocchi; M Ferraro
Journal:  Exp Cell Res       Date:  1978-09       Impact factor: 3.905

8.  Argyrophilic nuclear and nucleolar proteins of Xenopus laevis oocytes identified by gel electrophoresis.

Authors:  M A Williams; J A Kleinschmidt; G Krohne; W W Franke
Journal:  Exp Cell Res       Date:  1982-02       Impact factor: 3.905

9.  Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function.

Authors:  B Knibiehler; C Mirre; M Hartung; P Jean; A Stahl
Journal:  Cytogenet Cell Genet       Date:  1981

10.  Identification of a silver binding protein associated with the cytological silver staining of actively transcribing nucleolar regions.

Authors:  H R Hubbell; L I Rothblum; T C Hsu
Journal:  Cell Biol Int Rep       Date:  1979-10
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  16 in total

1.  Cytochemical variations in the nucleolus during spermiogenesis in man and monkey.

Authors:  J P Dadoune; M F Alfonsi; M A Fain-Maurel
Journal:  Cell Tissue Res       Date:  1991-04       Impact factor: 5.249

2.  Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level.

Authors:  D Ploton; M Menager; P Jeannesson; G Himber; F Pigeon; J J Adnet
Journal:  Histochem J       Date:  1986-01

3.  Three-dimensional organization of the ribosomal genes and Ag-NOR proteins during interphase and mitosis in PtK1 cells studied by confocal microscopy.

Authors:  I Robert-Fortel; H R Junéra; G Géraud; D Hernandez-Verdun
Journal:  Chromosoma       Date:  1993-02       Impact factor: 4.316

4.  A new version of the Ag-NOR technique. A combination with DAPI staining.

Authors:  S M Biliński; B Bilińska
Journal:  Histochem J       Date:  1996-09

5.  Genotoxicity evaluation of environmental pollutants using analysis of nucleolar alterations.

Authors:  Dânia Elisa Christofoletti Mazzeo; Maria Aparecida Marin-Morales
Journal:  Environ Sci Pollut Res Int       Date:  2015-02-03       Impact factor: 4.223

6.  Microwave irradiation improvements in the silver staining of the nucleolar organizer (Ag-NOR) technique.

Authors:  F J Medina; A Cerdido; R Marco
Journal:  Histochem Cell Biol       Date:  1995-06       Impact factor: 4.304

7.  Selective staining of the same set of nucleolar phosphoproteins by silver and Giemsa. A combined biochemical and cytochemical study on staining of NORs.

Authors:  C H Buys; J Osinga
Journal:  Chromosoma       Date:  1984       Impact factor: 4.316

8.  Number of nucleoli and coiled bodies and distribution of fibrillar centres in differentiating Purkinje neurons of chick and rat cerebellum.

Authors:  M Lafarga; M A Andrés; C Fernández-Viadero; J Villegas; M T Berciano
Journal:  Anat Embryol (Berl)       Date:  1995-04

9.  Simultaneous high resolution localization of Ag-NOR proteins and nucleoproteins in interphasic and mitotic nuclei.

Authors:  D Ploton; M Menager; J J Adnet
Journal:  Histochem J       Date:  1984-08

10.  Stereoscopic back-scattered electron imaging of silver-stained proteins in nucleoli.

Authors:  F Thiébaut; J P Rigaut; K Feren; A Reith
Journal:  Chromosoma       Date:  1985       Impact factor: 4.316
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