Identification of mononuclear cells in cell suspension from cutaneous infiltrates freed by a suction blister method.

Mononuclear cells were liberated from superficial skin inflammations by a suction blister method which yielded a sufficient number of cells for histochemical and immunocytochemical analysis. The cell population consisted mainly of T cells which were identified as sheep red blood cell rosettes. Very few B cells were found as rosettes with immunobeads. Macrophages and eosinophils were found in varying numbers and they were identified histochemically. The method is suitable for studying nontumorous skin infiltrates.