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Literature DB >> 6183447

Isolation of a monoclonal antibody viral polypeptide VP2 of simian virus 40.

Abstract

A stable hybridoma cell line, IIG8-203-2, that secretes a monoclonal antibody of the immunoglobulin subclass M was obtained by fusion of mouse myeloma cells with spleen cells of mice that had been immunized with the viral polypeptide VP2 of simian virus 40. The monoclonal antibody recognizes viral polypeptides that migrate with VP2 polypeptides in a sodium dodecyl sulfate-polyacrylamide gel. It also recognizes two intracellular polypeptides (29,000 and 37,000 daltons) in a detergent-insoluble fraction extracted 30 h after virus infection of TC7 cells.

Entities: Chemical Disease Gene Species

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Year: 1982 PMID： 6183447 PMCID： PMC256276

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

18 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors: G Köhler; C Milstein
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

2. Fingerprints of polyoma virus proteins and mouse histones.

Authors: G Fey; B Hirt
Journal: Cold Spring Harb Symp Quant Biol Date: 1975

3. Resolutions and identification of the core deoxynucleoproteins of the simian virus 40.

Authors: R S Lake; S Barban; N P Salzman
Journal: Biochem Biophys Res Commun Date: 1973-09-18 Impact factor: 3.575

4. Protein metabolism in SV40-infected cells.

Authors: E D Kiehn
Journal: Virology Date: 1973-11 Impact factor: 3.616

5. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells.

Authors: H L Ozer
Journal: J Virol Date: 1972-01 Impact factor: 5.103

6. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors: E Engvall; P Perlmann
Journal: J Immunol Date: 1972-07 Impact factor: 5.422

Isolation of a monoclonal antibody viral polypeptide VP2 of simian virus 40.

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

2. Fingerprints of polyoma virus proteins and mouse histones.

3. Resolutions and identification of the core deoxynucleoproteins of the simian virus 40.

4. Protein metabolism in SV40-infected cells.

5. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells.

6. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

7. Structural polypeptides of simian virus 40.

8. Identification of virus-induced proteins in cells productively infected with simian virus 40.

9. Protein synthesis in Simian virus 40-infected monkey cells.

10. Pattern of protein synthesis in monkey cells infected by simian virus 40.

1. Recognition of SV40-VP2 in the infected cell by antipeptide antibodies.

2. Cooperation of structural proteins during late events in the life cycle of polyomavirus.