Literature DB >> 6182964

A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain.

R S Sloviter.   

Abstract

A simplified sulphide/silver technique for visualizing transitional metals in rat brain is described. It consists of: (1) sulphide perfusion before formaldehyde perfusion, (2) routine paraffin embedding and sectioning, and (3) silver staining with physical developer. This method is compatible with formaldehyde fixation, eliminates the need for post-fixation in Carnoy's solution before embedding and does not require low paraffin temperatures during embedding. Once embedded, brains can be stored for months before sectioning without loss of stain quality.

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Year:  1982        PMID： 6182964     DOI： 10.1016/0361-9230(82)90104-6

Source DB:  PubMed          Journal:  Brain Res Bull        ISSN： 0361-9230            Impact factor:   4.077


  36 in total

1.  Dentate granule cell neurogenesis is increased by seizures and contributes to aberrant network reorganization in the adult rat hippocampus.

Authors:  J M Parent; T W Yu; R T Leibowitz; D H Geschwind; R S Sloviter; D H Lowenstein
Journal:  J Neurosci       Date:  1997-05-15       Impact factor: 6.167

2.  Manganese-enhanced magnetic resonance imaging detects mossy fiber sprouting in the pilocarpine model of epilepsy.

Authors:  Jackeline M Malheiros; Roberson S Polli; Fernando F Paiva; Beatriz M Longo; Luiz E Mello; Afonso C Silva; Alberto Tannús; Luciene Covolan
Journal:  Epilepsia       Date:  2012-05-29       Impact factor: 5.864

3.  Environmental lead exposure during early life alters granule cell neurogenesis and morphology in the hippocampus of young adult rats.

Authors:  T Verina; C A Rohde; T R Guilarte
Journal:  Neuroscience       Date:  2007-02-01       Impact factor: 3.590

4.  Borders and comparative cytoarchitecture of the perirhinal and postrhinal cortices in an F1 hybrid mouse.

Authors:  Stephane A Beaudin; Teghpal Singh; Kara L Agster; Rebecca D Burwell
Journal:  Cereb Cortex       Date:  2012-02-23       Impact factor: 5.357

5.  Genetic deletion and pharmacological inhibition of Nogo-66 receptor impairs cognitive outcome after traumatic brain injury in mice.

Authors:  Anders Hånell; Fredrik Clausen; Maria Björk; Kristine Jansson; Ola Philipson; Lars N G Nilsson; Lars Hillered; Paul H Weinreb; Daniel Lee; Tracy K McIntosh; David A Gimbel; Stephen M Strittmatter; Niklas Marklund
Journal:  J Neurotrauma       Date:  2010-07       Impact factor: 5.269

6.  Electron microscopic analysis of glial cells in the rat telencephalon stained with the Neo-Timm and selenium methods.

Authors:  I E Holm
Journal:  Histochemistry       Date:  1989

7.  Transection of CA3 does not affect memory performance in rats.

Authors:  Mohamad Z Koubeissi; Saifur Rashid; Gemma Casadesus; Kui Xu; Tanvir U Syed; Hans Lüders; Dominique Durand
Journal:  Epilepsy Behav       Date:  2011-05-14       Impact factor: 2.937

8.  Hippocampal zinc infusion delays the development of afterdischarges and seizures in a kindling model of epilepsy.

Authors:  Siegward-M Elsas; Saman Hazany; William L Gregory; Istvan Mody
Journal:  Epilepsia       Date:  2009-01-26       Impact factor: 5.864

9.  Temperature effect on immunostaining of microtubule-associated protein 2 and synaptophysin after 30 minutes of forebrain ischemia in rat.

Authors:  T Miyazawa; P Bonnekoh; K A Hossmann
Journal:  Acta Neuropathol       Date:  1993       Impact factor: 17.088

10.  Altered longevity-assurance activity of p53:p44 in the mouse causes memory loss, neurodegeneration and premature death.

Authors:  Mariana Pehar; Kenneth J O'Riordan; Melissa Burns-Cusato; Matthew E Andrzejewski; Carlos Gil del Alcazar; Corinna Burger; Heidi Scrable; Luigi Puglielli
Journal:  Aging Cell       Date:  2010-04       Impact factor: 9.304
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