Differential staining of chromosomes and spindle and its use as an assay for determining the effect of diethylstilboestrol on cultured mammalian cells.

A differential staining technique that allows for the simultaneous visualization of chromosome and spindle fibres has been used to investigate the action of diethylstilboestrol (DES) on cultured human fibroblasts. It was found that the mitotic index increased while spindle-fibre formation was inhibited with increasing DES concentration. Normal cellular division was severely affected by DES exposure in a dose-related manner. It has been demonstrated that this new staining technique may help to show up some mitotic poisons which are an important group currently missed by bacterial and clastogenic tests.