A study of granulocyte cytotoxins and detection of granulocyte allospecific antigens.

Granulocytes were isolated b overlaying leukocyte-rich plasma discontinuous density gradients. The cell suspensions were 95 percent granulocytes. Sera of 383 polytransfused patients and 521 multiparous women were examined for lymphocytotoxins, granulocytotoxins, and granulocytoagglutinins. On the basis of these tests, sera were identified which contained only granulocytotoxins. These were further identified with more precision as to their titers and antibody absorption; reactivity with granulocytes, B, and T lymphocytes; and K 562 cell line. On the basis of calculated correlation coefficients, nine sera were selected which determined three granulocyte specificities having genotype frequencies consistent with the Hardy-Weinberg equilibrium.