Literature DB >> 6181257

T-cell hybridoma production of macrophage activation factor (MAF) I. Separation of MAF from interferon gamma.

T L Ratliff, D L Thomasson, R E McCool, W J Catalona.   

Abstract

A T cell hybridoma was developed that produced macrophage activation factor (MAF) but no gamma interferon (IFN gamma). Hybridoma MAF was produced after stimulation with either concanavalin A (Con A) or phytohemagglutinin (PHA). Dose-response experiments showed that 1.5 microgram/ml Con A provided maximum MAF production with equivalent levels of MAF produced at Con A concentrations up to 6 microgram/ml. Time-course studies showed that maximum MAF activity was observed 48 hours after mitogen stimulation, and titration of MAF-containing supernatants showed that maximal MAF activity was observed at 1:4 dilutions with significant activity present at dilutions as great as 1:64. No IFN gamma activity was detectable at any supernatant dilution.

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Year:  1982        PMID： 6181257

Source DB:  PubMed          Journal:  J Reticuloendothel Soc        ISSN： 0033-6890


  6 in total

1.  Potentiation of direct antitumor cytotoxicity and production of tumor cytolytic factors in human blood monocytes by human recombinant interferon-gamma and muramyl dipeptide derivatives.

Authors:  S Sone; G Lopez-Berestein; I J Fidler
Journal:  Cancer Immunol Immunother       Date:  1986       Impact factor: 6.968

2.  Studies on macrophage-activating factor (MAF) in antitumor immune responses. II. Molecular characterization of MAF produced by the tumor-immune Lyt-1+2- T cell subset.

Authors:  H Nakajima; Y Izumi; S Sugihara; Y Satoh; S Isumi; T Gotoh; H Fujiwara; T Hamaoka
Journal:  Cancer Immunol Immunother       Date:  1987       Impact factor: 6.968

3.  Mechanism(s) of in vitro macrophage activation with Nocardia rubra cell wall skeleton: the effects on macrophage activating factor production by lymphocytes.

Authors:  T Masuno; S Hayashi; M Ito; T Ikeda; T Ogura; S Kishimoto; Y Yamamura
Journal:  Cancer Immunol Immunother       Date:  1986       Impact factor: 6.968

4.  T-cell hybridoma-produced lymphokine that activates macrophages to suppress intracellular growth of Histoplasma capsulatum.

Authors:  B Wu-Hsieh; A Zlotnik; D H Howard
Journal:  Infect Immun       Date:  1984-01       Impact factor: 3.441

5.  Recombinant and natural gamma-interferon activation of macrophages in vitro: different dose requirements for induction of killing activity against phagocytizable and nonphagocytizable fungi.

Authors:  E Brummer; C J Morrison; D A Stevens
Journal:  Infect Immun       Date:  1985-09       Impact factor: 3.441

6.  Macrophage activation: priming activity from a T-cell hybridoma is attributable to interferon-gamma.

Authors:  J L Pace; S W Russell; R D Schreiber; A Altman; D H Katz
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205
  6 in total

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