| Literature DB >> 6181090 |
B F Anthony, N F Concepcion, S A McGeary, J I Ward, D C Heiner, P Shapshak, R A Insel.
Abstract
Type-specific antigen was purified from the supernatant of type III group B streptococcal cultures, tyrosylated, and bound to microtiter wells for an enzyme-linked immunosorbent assay. The immunological specificity of the antigen and the assay was shown by (i) reaction only with homologous unabsorbed rabbit sera and (ii) inhibition after incubation of human serum with homologous but not heterologous purified antigen. The assay was quantitated by relating optical density readings to absolute amounts of human immunoglobulin G bound to the microtiter wells.Entities:
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Year: 1982 PMID: 6181090 PMCID: PMC272359 DOI: 10.1128/jcm.16.2.350-354.1982
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948