| Literature DB >> 6180996 |
T A Bewley, H L Levine, R Wetzel.
Abstract
The solution structure of human leukocyte (alpha) interferon-A (LeIF-A) purified from E. coli extracts was investigated by circular dichroism spectroscopy. At pH 8.2, the native molecule exhibits 40-70% alpha-helix and no clearly detectable beta-structure. The tertiary structure includes a closely-packed interior containing at least one tryptophan side-chain. Titration to pH 1.5 produces a partial loss of structural features which are rapidly regained on return to neutral pH.Entities:
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Year: 1982 PMID： 6180996 DOI： 10.1111/j.1399-3011.1982.tb02658.x
Source DB: PubMed Journal: Int J Pept Protein Res ISSN： 0367-8377