Literature DB >> 6180489

Somatic cell genetic analysis of HLA-A, B, C and human beta 2-microglobulin expression.

M E Kamarck, J A Barbosa, F H Ruddle.   

Abstract

We have examined the cell surface expression of the human histocompatibility antigens HLA-A, B, C and beta 2-microglobulin (beta 2m) on a human-mouse somatic cell hybrid line. Using specific antibodies and the fluorescence-activated cell sorter (FACS), we viably fractionated and characterized four separate hybrid subpopulations (HLA+,beta 2m+; HLA+,beta 2m-; HLA-,beta 2m+; HLA-,beta 2m-). Hybrid selection based on surface antigen expression resulted in corresponding genetic selection for and against human chromosomes 6 and 15. Studies of the homogeneous hybrid sublines revealed that the presence of human beta 2m in a hybrid cell dramatically increased the surface expression of human HLA-A, B, C and mouse H-2Kk antigens. The results demonstrate the importance of human chromosome-specific surface markers and the fluorescence-activated cell sorter in somatic cell genetic analysis.

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Year:  1982        PMID： 6180489     DOI： 10.1007/bf01538895

Source DB:  PubMed          Journal:  Somatic Cell Genet        ISSN： 0098-0366


  12 in total

1.  Molecular cloning, expression, and chromosomal localization of the gene encoding a human myeloid membrane antigen (gp150).

Authors:  A T Look; S C Peiper; M B Rebentisch; R A Ashmun; M F Roussel; R S Lemons; M M Le Beau; C M Rubin; C J Sherr
Journal:  J Clin Invest       Date:  1986-10       Impact factor: 14.808

2.  Carcinoembryonic antigen family: expression in a mouse L-cell transfectant and characterization of a partial cDNA in bacteriophage lambda gt11.

Authors:  M E Kamarck; J J Elting; J T Hart; S J Goebel; P M Rae; M A Nothdurft; J J Nedwin; T R Barnett
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

3.  Amino acid sequences in the alpha 1 domain and not glycosylation are important in HLA-A2/beta 2-microglobulin association and cell surface expression.

Authors:  J Santos-Aguado; P A Biro; U Fuhrmann; J L Strominger; J A Barbosa
Journal:  Mol Cell Biol       Date:  1987-03       Impact factor: 4.272

4.  Recognition of HLA-A2 and -B7 antigens by cloned cytotoxic T lymphocytes after gene transfer into human and monkey, but not mouse, cells.

Authors:  J A Barbosa; S J Mentzer; G Minowada; J L Strominger; S J Burakoff; P A Biro
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

5.  Identification of human genomic clones coding the major histocompatibility antigens HLA-a2 and HLA-B7 by DNA-mediated gene transfer.

Authors:  J A Barbosa; M E Kamarck; P A Biro; S M Weissman; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

6.  High-level expression of a cloned HLA heavy chain gene introduced into mouse cells on a bovine papillomavirus vector.

Authors:  D DiMaio; V Corbin; E Sibley; T Maniatis
Journal:  Mol Cell Biol       Date:  1984-02       Impact factor: 4.272

7.  Characterization of a human X mouse T cell hybridoma and identification of a clone secreting and binding interleukin-2.

Authors:  L G Durrant; M Parkar; N Kenworthy; G M Taylor
Journal:  Immunology       Date:  1984-05       Impact factor: 7.397

8.  Cloning, sequence analysis, and expression of the large subunit of the human lymphocyte activation antigen 4F2.

Authors:  J A Lumadue; A B Glick; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

9.  Hormonal regulation of cell surface expression of the major histocompatibility antigen H-2Ld in transfected cells.

Authors:  H Ponta; R Ball; M Steinmetz; B Groner
Journal:  EMBO J       Date:  1985-12-16       Impact factor: 11.598

10.  Somatic variants of the level of expression of a cell surface antigen.

Authors:  O R Burrone; F Calabi; R F Kefford; C Milstein
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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