| Literature DB >> 6180061 |
J E Whitman, G M Crowley, J Tou, J V Tredway, C l Hung.
Abstract
Human lymphoblastoid cell-derived interferon-alpha has been purified by a combination of chromatography on controlled-pore glass 350, gel filtration on Ultrogel AcA 54 and hydrophobic chromatography on Phenyl-Sepharose CL-4B. Specific activities of greater than 10(7) units/mg have been obtained. Controlled-Pore Glass has the advantages of accommodating large volumes of induced medium in an "in-line" process giving high recoveries of interferon and removing most of any contaminating DNA.Entities:
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Year: 1981 PMID： 6180061 DOI： 10.1089/jir.1981.1.305
Source DB: PubMed Journal: J Interferon Res ISSN： 0197-8357