| Literature DB >> 6179256 |
R Billing, B Clark, K Lucero, B J Shi, P Terasaki.
Abstract
Spleen cells from a mouse immunized with an AML cell expressing HLA-A3 produced a hybridoma secreting an anti-HLA-A3, A11 monoclonal antibody, 26D3. By complement-dependent cytotoxicity at dilutions to 1:10(4) the ascites antibody lysed 13/13 A3, 15/15 A11, and 8 other lymphocytes from a panel of 98 donors. The 26 D3 immunoprecipitated a molecule consisting of subunits of 44,000 and 12,000 daltons. Monoclonality of the antibody was demonstrated by isoelectric focusing and protein A affinity chromatography.Entities:
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Year: 1982 PMID： 6179256 DOI： 10.1111/j.1399-0039.1982.tb01427.x
Source DB: PubMed Journal: Tissue Antigens ISSN： 0001-2815