Immunochemical characterization of binding sites of hybridoma antibodies specific for alpha (1 leads to 6) linked dextran.

The combining sites of seven BALB/c IgM, four BALB/c IgA and one C57BL/6 IgA hybridoma antibodies specific for alpha (1 leads to 6) linked dextran were probed by precipitin and precipitin inhibition assays. The 12 antibodies are able to bind to linear determinants in the interior of the dextran molecule; some have sites complementary to six alpha (1 leads to 6) linked glucose residues and other have sites complementary to seven alpha (1 leads to 6) linked glucose residues. From the analysis of the precipitins and precipitin inhibitions, it is concluded that no two hybridoma proteins have identical binding sites.