Literature DB >> 6178684

Antibodies to sperm-whale myoglobin evoked by free synthetic peptides of an antigenic site.

C R Young, M Z Atassi.   

Abstract

Previous studies from this laboratory have resulted in the determination of the entire antigenic structure of myoglobin (Mb). The present work was carried out to investigate the antigenicity of the synthetic antigenic sites of Mb in their free form (i.e. without coupling to a carrier) and the effect of peptide size on antigenicity. Site 5 (peptide 145-151) and the longer peptides 145-153, 143-153 and 132-153, each of which carries within it the region of site 5, were synthesized. Immunization of three different mouse strains with each of these peptides, in their free form, resulted in the formation of antibodies that bound specifically to intact Mb. The advantages and significance of these findings are discussed.

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Year:  1982        PMID： 6178684     DOI： 10.3109/08820138209050719

Source DB:  PubMed          Journal:  Immunol Commun        ISSN： 0090-0877


  12 in total

1.  Effects of amino acid substitutions outside an antigenic site on protein binding to monoclonal antibodies of predetermined specificity obtained by peptide immunization: demonstration with region 56-62 (antigenic site 2) of myoglobin.

Authors:  M S Abaza; M Z Atassi
Journal:  J Protein Chem       Date:  1992-10

2.  Effects of amino acid substitutions outside an antigenic site on protein binding to monoclonal antibodies of predetermined specificity obtained by peptide immunization: demonstration with region 113-120 (antigenic site 4) of myoglobin.

Authors:  M S Abaza; M Z Atassi
Journal:  J Protein Chem       Date:  1992-12

3.  Generation of species-specific antihemoglobin antibodies by immunization with synthetic peptides of human hemoglobin.

Authors:  M Oshima; M Z Atassi
Journal:  J Protein Chem       Date:  1989-12

4.  Analysis of exposed regions on the main extracellular domain of mouse acetylcholine receptor alpha subunit in live muscle cells by binding profiles of antipeptide antibodies.

Authors:  K Jinnai; T Ashizawa; M Z Atassi
Journal:  J Protein Chem       Date:  1994-11

5.  Amino acid substitutions outside a preselected antigenic region in hemoglobin affect the binding to monoclonal antibodies obtained by immunization with the synthetic region.

Authors:  M Oshima; S Nakamura; M Z Atassi
Journal:  J Protein Chem       Date:  1993-08

6.  Towards new vaccines.

Authors:  C R Young
Journal:  J R Soc Med       Date:  1984-04       Impact factor: 5.344

7.  Mapping the extracellular topography of the alpha-chain in free and in membrane-bound acetylcholine receptor by antibodies against overlapping peptides spanning the entire extracellular parts of the chain.

Authors:  M Z Atassi; B Mulac-Jericevic
Journal:  J Protein Chem       Date:  1994-01

8.  Immunogenicity of heme complexes of peptides designed to mimic the heme environment of myoglobin and hemoglobin.

Authors:  M Zouhair Atassi; Catherine Childress
Journal:  Protein J       Date:  2005-01       Impact factor: 2.371

9.  Effects of amino acid substitutions outside an antigenic site on protein binding to monoclonal antibodies of predetermined specificity obtained by peptide immunization: demonstration with region 15-22 (antigenic site 1) of myoglobin.

Authors:  M S Abaza; C R Young; M Z Atassi
Journal:  J Protein Chem       Date:  1992-10

10.  Effects of amino acid substitutions outside an antigenic site on protein binding to monoclonal antibodies of predetermined specificity obtained by peptide immunization: demonstration with region 94-100 (antigenic site 3) of myoglobin.

Authors:  M S Abaza; M Z Atassi
Journal:  J Protein Chem       Date:  1992-10
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