Literature DB >> 6178023

Measurement of SCE frequencies in plants: a simple Feulgen-staining procedure for vicia faba.

M J Tempelaar, M T de Both, J E Versteegh.   

Abstract

A relatively straightforward approach is described to obtain differential contrast in sister chromatids for SCE detection in Vicia faba after BrdUrd incorporation. The hydrolysis time of the well-known Feulgen reaction was extended to differentially degrade the DNA, the BrdUrd-substituted strands being more resistant. The procedure may easily be adapted for other plant species with large chromosomes.

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Year:  1982        PMID: 6178023     DOI: 10.1016/0165-7992(82)90061-6

Source DB:  PubMed          Journal:  Mutat Res        ISSN: 0027-5107            Impact factor:   2.433


  2 in total

1.  Ploidy levels in leaf callus and regenerated plants of Solanum tuberosum determined by cytophotometric measurements of protoplasts.

Authors:  E Jacobsen; M J Tempelaar; E W Bijmolt
Journal:  Theor Appl Genet       Date:  1983-05       Impact factor: 5.699

2.  Fusion characteristics of plant protoplasts in electric fields.

Authors:  M J Tempelaar; M G Jones
Journal:  Planta       Date:  1985-08       Impact factor: 4.116

  2 in total

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