The ultrastructure of the subnucleus gelatinosus of the nucleus of the tractus solitarius in the cat.

The dorsomedial region of the nucleus of the tractus solitarius termed the subnucleus gelatinosus (SNG) was studied at the light and electron microscopic level in the cat. In cresyl violet and luxol fast blue stained sections the SNG contained small neuronal somata that were scattered throughout a pale-staining neuropil containing few myelinated fibers. These neurons were difficult to impregnate with Golgi staining techniques, but in successful impregnations the somata were observed to be 10--19 micrometers in diameter and bore few sparsely branching primary dendrites. Spines were present on the dendrites of some neurons and were more numerous on distal portions of the dendritic tree. Ultrastructural examination of the SNG revealed that the neuronal complement consisted of round, oval, or spindle shaped neurons with little or no organized Nissl substance. Rare myelin-like ensheathments of neuronal perikarya were also observed. Bundles of fine unmyelinated axons that coursed mainly longitudinally were a prominent feature of the area. The most common type of axon terminal observed contained mainly round clear vesicles, approximately 31 nm in diameter, and made asymmetrical synaptic contact with a dendritic profile. Pleomorphic vesicle-containing terminals involved in symmetrical synaptic contact were also commonly seen. Axodendritic and axosomatic synapses were associated with terminals containing either round clear vesicles or pleomorphic vesicles. Less commonly, dendrodendritic and dendrosomatic synapses were seen, the presynaptic elements of which contained pleomorphic vesicles. Following removal of a nodose ganglion, degenerating terminals of vagal afferent fibers were observed throughout the neuropil. Such terminals contained round, clear vesicles with an occasional large, dense-cored vesicle, and made axodendritic and axosomatic synaptic contacts.