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Literature DB >> 6177623

DE-52 and DE-53 cellulose microcarriers. I. Growth of primary and established anchorage-dependent cells.

S Reuveny, L Silberstein, A Shahar, E Freeman, A Mizrahi.

Abstract

DEAE cellulose anion exchangers having small ion exchange capacity (0.5 to 2.0 meq/g dry material) were tested for cell attachment kinetics and capacity to support growth anchorage-dependent cells. It was found that cells from established cell lines (BHK and MDCK) can grow to confluency on DEAE cellulose particles having exchange capacity of 1 and 2 meq/g dry materials, DE-52 and DE-53, respectively. On the other hand, chick embryo fibroblasts (primary cells) can grow only on DE-53 particles.

Entities: Chemical Species

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Year: 1982 PMID： 6177623 DOI： 10.1007/BF02796400

Source DB: PubMed Journal: In Vitro ISSN： 0073-5655

11 in total

1. A simple method of preparing a cell suspension for scanning electron microscopy.

Authors: G E Jones; R Gillett
Journal: Experientia Date: 1975-10-15

2. The submerged culture of mammalian cells; the spinner culture.

Authors: W F McLIMANS; E V DAVIS; F L GLOVER; G W RAKE
Journal: J Immunol Date: 1957-11 Impact factor: 5.422

3. The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of DEAE sephadex A50 beads.

Authors: R E Spier; J P Whiteside
Journal: Biotechnol Bioeng Date: 1976-05 Impact factor: 4.530

DE-52 and DE-53 cellulose microcarriers. I. Growth of primary and established anchorage-dependent cells.

1. A simple method of preparing a cell suspension for scanning electron microscopy.

2. The submerged culture of mammalian cells; the spinner culture.

3. The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of DEAE sephadex A50 beads.

4. The measurement of proliferation in tissue cultures by enumeration of cell nuclei.

5. Chemical and mechanical requirements for fibroblast adhesion.

6. Effects of cell adhesion of the substratum on the growth of chick embryo fibroblasts.

7. Growth of cell-strains and primary cells on micro-carriers in homogeneous culture.

8. Homogeneous cultivation of animal cells for the production of virus and virus products.

9. Microcarrier cell culture: new methods for research-scale application.

10. A new cellulose-based microcarrier culturing system.

1. Differentiation of myoblasts and CNS cells grown either separately or as co-cultures on microcarriers.

2. Microcarrier technology, present status and perspective.

3. Pure gelatin microcarriers: synthesis and use in cell attachment and growth of fibroblast and endothelial cells.