Literature DB >> 6174944

Molecular cloning and analysis of cDNA sequences derived from poly A+ RNA from barley endosperm: identification of B hordein related clones.

B G Forde, M Kreis, M B Bahramian, J A Matthews, B J Miflin, R D Thompson, D Bartels, R B Flavell.   

Abstract

A collection of over 130 cDNA clones has been constructed in the bacterial plasmids pPH207 and pBR322 using as template the poly A+ RNA from membrane-bound polysomes of barley endosperm (cv. Sundance). Fifty four B hordein cDNA clones have been identified by cross-hybridization analysis and in vitro translation of plasmid-selected mRNAs. Hybridization of 11 of the B hordein cDNA clones to Northern blots of size-fractionated RNA indicated that the B hordein mRNA is ca. 1300 nucleotides long. One cDNA clone, pHvE-c16, has been partially sequenced and shown by comparison with C-terminal and other peptide sequences to be related to B1 hordein polypeptides. The results obtained from the analysis of the B hordein cDNA clones support the idea that the Hor 2 locus, which specifies the B hordeins, is complex and codes for a family of related mRNA species.

Entities:  

Mesh：

Substances：

Year:  1981        PMID： 6174944      PMCID： PMC327634          DOI： 10.1093/nar/9.24.6689

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

1.  Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors:  J M Bailey; N Davidson
Journal:  Anal Biochem       Date:  1976-01       Impact factor: 3.365

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors:  J P Chamberlain
Journal:  Anal Biochem       Date:  1979-09-15       Impact factor: 3.365

6.  Electrophoretic fractionation and translation in vitro of poly(rA)-containing RNA from maize endosperm. Evidence of two mRNAs coding for zein protein.

Authors:  U Wienand; G Feix
Journal:  Eur J Biochem       Date:  1978-12

7.  Cloning and characterization of ribosomal RNA genes from wheat and barley.

Authors:  W L Gerlach; J R Bedbrook
Journal:  Nucleic Acids Res       Date:  1979-12-11       Impact factor: 16.971

8.  A cDNA clone from Zea mays endosperm sucrose synthetase mRNA.

Authors:  M Geiser; H P Döring; J Wöstemeyer; U Behrens; E Tillmann; P Starlinger
Journal:  Nucleic Acids Res       Date:  1980-12-20       Impact factor: 16.971

9.  Recombinant plasmids containing avian vitellogenin structural gene sequences derived from complementary DNA.

Authors:  T Ohno; P J Cozens; A C Cato; J P Jost
Journal:  Biochim Biophys Acta       Date:  1980

10.  The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hor-2 locus in barley (Hordeum vulgare L).

Authors:  A J Faulks; P R Shewry; B J Miflin
Journal:  Biochem Genet       Date:  1981-10       Impact factor: 1.890
View more
  41 in total

1.  Methylation of B-hordein genes in barley endosperm is inversely correlated with gene activity and affected by the regulatory gene Lys3.

Authors:  M B Sørensen
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

2.  Seasonal changes in the concentration of the major storage protein and its mRNA in xylem ray cells of poplar trees.

Authors:  S Clausen; K Apel
Journal:  Plant Mol Biol       Date:  1991-10       Impact factor: 4.076

3.  Molecular cloning of pea mRNAs encoding a shoot-specific polypeptide and light-induced polypeptides.

Authors:  S C De Vries; M C Harmsen; M T Kuiper; H J Dons; J G Wessels
Journal:  Plant Mol Biol       Date:  1983-11       Impact factor: 4.076

4.  Identification and DNA sequence analysis of a γ-type gliadin cDNA plasmid from winter wheat.

Authors:  T W Okita
Journal:  Plant Mol Biol       Date:  1984-11       Impact factor: 4.076

5.  The Anticyclic Timing of Leaf Senescence in the Parasitic Plant Viscum album Is Closely Correlated with the Selective Degradation of Sulfur-Rich Viscotoxins.

Authors:  G. Schrader-Fischer; K. Apel
Journal:  Plant Physiol       Date:  1993-03       Impact factor: 8.340

6.  Acquired Resistance in Barley (The Resistance Mechanism Induced by 2,6-Dichloroisonicotinic Acid Is a Phenocopy of a Genetically Based Mechanism Governing Race-Specific Powdery Mildew Resistance).

Authors:  K. H. Kogel; U. Beckhove; J. Dreschers; S. Munch; Y. Romme
Journal:  Plant Physiol       Date:  1994-12       Impact factor: 8.340

7.  Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat and maize.

Authors:  B G Forde; A Heyworth; J Pywell; M Kreis
Journal:  Nucleic Acids Res       Date:  1985-10-25       Impact factor: 16.971

8.  cDNA-derived identification of novel thionin precursors in Viscum album that contain highly divergent thionin domains but conserved signal and acidic polypeptide domains.

Authors:  G Schrader-Fischer; K Apel
Journal:  Plant Mol Biol       Date:  1993-12       Impact factor: 4.076

9.  Molecular analysis of the effects of the lys 3a gene on the expression of Hor loci in developing endosperms of barley (Hordeum vulgare L.).

Authors:  M Kreis; P R Shewry; B G Forde; S Rahman; M B Bahramian; B J Miflin
Journal:  Biochem Genet       Date:  1984-04       Impact factor: 1.890

10.  Ribosome-deficient plastids affect transcription of light-induced nuclear genes: genetic evidence for a plastid-derived signal.

Authors:  W R Hess; A Müller; F Nagy; T Börner
Journal:  Mol Gen Genet       Date:  1994-02
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.