Literature DB >> 6174113

Visualization of isozymes which generate inorganic phosphate.

R J Klebe, S Schloss, L Mock, C R Link.   

Abstract

A sensitive, new enzymatic method for the detection of isozymes which liberate inorganic phosphate (or pyrophosphate) is described. The new method for the detection of phosphate differs from the established method for nucleoside phosphorylase by the substitution of one reagent. The new enzymatic method, compared to existing methods for the detection of phosphate on electrophoretic gels, is advantageous due to its sensitivity and generation of a nondiffusible formazan chromogenic product.

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Year:  1981        PMID： 6174113     DOI： 10.1007/BF00504257

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  11 in total

1.  A simple method for the quantitation of isozyme patterns.

Authors:  R J Klebe
Journal:  Biochem Genet       Date:  1975-12       Impact factor: 1.890

2.  EFFECTS OF ADENYLIC ACID ON THE KINETICS OF MUSCLE PHOSPHORYLASE A.

Authors:  O H LOWRY; D W SCHULZ; J V PASSONNEAU
Journal:  J Biol Chem       Date:  1964-06       Impact factor: 5.157

3.  Purine nucleoside phosphorylase from human erythrocytes.

Authors:  J D Stoeckler; R P Agarwal; K C Agarwal; R E Parks
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

4.  Modifications in the enzymatic assay for inorganic phosphate.

Authors:  N W Cornell; M G Leadbetter; R L Veech
Journal:  Anal Biochem       Date:  1979-06       Impact factor: 3.365

5.  Studies on human erythrocyte inorganic pyrophosphatase.

Authors:  R A Fisher; B M Turner; H L Dorkin; H Harris
Journal:  Ann Hum Genet       Date:  1974-01       Impact factor: 1.670

6.  Purification and specificity of cellobiose phosphorylase from Clostridium thermocellum.

Authors:  J K Alexander
Journal:  J Biol Chem       Date:  1968-06-10       Impact factor: 5.157

7.  Inherited variants of human nucleoside phosphorylase.

Authors:  Y H Edwards; D A Hopkinson; H Harris
Journal:  Ann Hum Genet       Date:  1971-05       Impact factor: 1.670

8.  Two-dimensional spectroscopy of electrophoretic gels.

Authors:  R J Klebe; M G Mancuso; C R Brown; L Teng
Journal:  Biochem Genet       Date:  1981-08       Impact factor: 1.890

9.  Bioautography: a general method for the visualization of isozymes.

Authors:  S L Naylor; R J Klebe
Journal:  Biochem Genet       Date:  1977-12       Impact factor: 1.890

10.  Nucleoside-phosphorylase deficiency in a child with severely defective T-cell immunity and normal B-cell immunity.

Authors:  E R Giblett; A J Ammann; D W Wara; R Sandman; L K Diamond
Journal:  Lancet       Date:  1975-05-03       Impact factor: 79.321
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