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Literature DB >> 6173732

A cis-acting regulator of enzyme tissue specificity in Drosophila is expressed at the RNA level.

Abstract

Alcohol dehydrogenase (ADH) is expressed according to qualitatively different patterns in Drosophila grimshawi and two closely related species, D. orthofascia and D. formella. This regulatory difference is under control of a cis-acting genetic factor. The ADH of these species is very similar to ADH of D. melanogaster and a genomic ADH clone derived from the latter hybridizes specifically to ADH coding sequences of the other species. Using this hybridization probe, it is shown that the above cis-acting regulator acts before or shortly after transcription to control ADH expression.

Entities: Species

Mesh：

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Year: 1981 PMID： 6173732 DOI： 10.1007/BF00270627

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

29 in total

1. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

Authors: J M Bailey; N Davidson
Journal: Anal Biochem Date: 1976-01 Impact factor: 3.365

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Regulation of the tissue specificity of an enzyme by a cis-acting genetic element: evidence from interspecific Drosophila hybrids.

Authors: W J Dickinson; H L Carson
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors: W M Bonner; R A Laskey
Journal: Eur J Biochem Date: 1974-07-01

5. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

A cis-acting regulator of enzyme tissue specificity in Drosophila is expressed at the RNA level.

1. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Regulation of the tissue specificity of an enzyme by a cis-acting genetic element: evidence from interspecific Drosophila hybrids.

4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

5. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

6. Drosophila alcohol dehydrogenase. Purification and partial characterization.

7. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

8. An efficient mRNA-dependent translation system from reticulocyte lysates.

9. Genetic control of heat sensitivity and activity level of murine arylsulfatase B.

10. Genetic determination of the beta-galactosidase developmental program in mouse liver.

1. Developmental control of transduced dopa decarboxylase genes in D. melanogaster.

2. Naturally occurring quantitative variants of acid phosphatase-1 in Drosophila melanogaster.