Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Stimulation in vitro of a heparin-resistant RNA polymerase I transcription complex.

Literature DB >> 6173040

Stimulation in vitro of a heparin-resistant RNA polymerase I transcription complex.

Abstract

A soluble factor partially purified from calf liver increases transcription by RNA polymerase I in isolated nuclei. Addition of the factor to reactions which have reached a plateau owing to the inability to reinitiate on the endogenous chromatin template restores the initial rate of synthesis and stimulates an increased accumulation of RNA product. The RNA synthesis stimulated by factor addition is identical with that initiated in vivo in that it is resistant to heparin disruption.

Entities: Species

Mesh：

Substances：

Year: 1981 PMID： 6173040 PMCID： PMC1163228 DOI： 10.1042/bj1980207

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

21 in total

1. Studies on the control of ribosomal RNA synthesis in HeLa cells.

Authors: C J Chesterton; B E Coupar; P H Butterworth; M H Green
Journal: Eur J Biochem Date: 1975-09-01

2. Transcription of specific genes in isolated nuclei from HeLa cells in vitro.

Authors: A Udvardy; K H Seifart
Journal: Eur J Biochem Date: 1976-02-16

3. Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.

Authors: I Grummt; V A Smith; F Grummt
Journal: Cell Date: 1976-03 Impact factor: 41.582

4. A short lived polypeptide component of one of two discrete functional pools of hepatic nuclear alpha-amanitin resistant RNA polymerases.

Authors: A Lampert; P Feigelson
Journal: Biochem Biophys Res Commun Date: 1974-06-18 Impact factor: 3.575

1. Microinjection of purified ornithine decarboxylase into Xenopus oocytes selectively stimulates ribosomal RNA synthesis.

Authors: D H Russell
Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205

1 in total

Stimulation in vitro of a heparin-resistant RNA polymerase I transcription complex.

1. Studies on the control of ribosomal RNA synthesis in HeLa cells.

2. Transcription of specific genes in isolated nuclei from HeLa cells in vitro.

3. Amino acid starvation affects the initiation frequency of nucleolar RNA polymerase.

4. A short lived polypeptide component of one of two discrete functional pools of hepatic nuclear alpha-amanitin resistant RNA polymerases.

5. Pre-4S RNA made in isolated HeLa cell nuclei terminates with U.

6. Comparative effect of heparin on RNA synthesis of isolated rat-liver nucleoli and purified RNA polymerase A.

7. Purification of rat liver and mouse ascites DNA-dependent RNA polymerase I.

8. Regulation of RNA polymerase I activity by ornithine decarboxylase.

9. Nuclei from rat liver: isolation method that combines purity with high yield.

10. Ornithine decarboxylase may function as an initiation factor for RNA polymerase I.

1. Microinjection of purified ornithine decarboxylase into Xenopus oocytes selectively stimulates ribosomal RNA synthesis.