Literature DB >> 6172049

Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB.

J B Toll, J E Wikberg, R G Andersson.   

Abstract

This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50--95% and the recovery was 30--40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests.

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Year:  1981        PMID: 6172049     DOI: 10.1111/j.1398-9995.1981.tb01847.x

Source DB:  PubMed          Journal:  Allergy        ISSN: 0105-4538            Impact factor:   13.146


  1 in total

1.  Studies of the mechanism of desensitization of anti-IgE-mediated histamine release from human basophils.

Authors:  R G Andersson; M Bartonek; E Lindström; I M Lindström; J B Toll
Journal:  Agents Actions       Date:  1989-04
  1 in total

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