Literature DB >> 6171588

A highly sensitive technique for staining DNA and RNA in polyacrylamide gels using silver.

T Boulikas, R Hancock.   

Abstract

A technique is described for staining DNA in polyacrylamide gels with silver. It is rapid, requiring about 30 min for whole staining and development procedure, very simple and at least 20 times more sensitive than ethidium bromide for the staining of double-stranded DNA in polyacrylamide gels. This technique can also be applied for the staining of denatured, single-stranded DNA as well as RNA after their electrophoretic separation on polyacrylamide gels, having the same sensitivity as for double-stranded DNA fragments.

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Year:  1981        PMID： 6171588     DOI： 10.1016/0165-022x(81)90046-4

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  7 in total

1.  Type II restriction endonucleases cleave single-stranded DNAs in general.

Authors:  K Nishigaki; Y Kaneko; H Wakuda; Y Husimi; T Tanaka
Journal:  Nucleic Acids Res       Date:  1985-08-26       Impact factor: 16.971

2.  The avian influenza virus nucleoprotein gene and a specific constellation of avian and human virus polymerase genes each specify attenuation of avian-human influenza A/Pintail/79 reassortant viruses for monkeys.

Authors:  M H Snyder; A J Buckler-White; W T London; E L Tierney; B R Murphy
Journal:  J Virol       Date:  1987-09       Impact factor: 5.103

3.  Four viral genes independently contribute to attenuation of live influenza A/Ann Arbor/6/60 (H2N2) cold-adapted reassortant virus vaccines.

Authors:  M H Snyder; R F Betts; D DeBorde; E L Tierney; M L Clements; D Herrington; S D Sears; R Dolin; H F Maassab; B R Murphy
Journal:  J Virol       Date:  1988-02       Impact factor: 5.103

4.  Nucleoprotein and membrane protein genes are associated with restriction of replication of influenza A/Mallard/NY/78 virus and its reassortants in squirrel monkey respiratory tract.

Authors:  S F Tian; A J Buckler-White; W T London; L J Reck; R M Chanock; B R Murphy
Journal:  J Virol       Date:  1985-03       Impact factor: 5.103

5.  Rescue of an influenza A virus wild-type PB2 gene and a mutant derivative bearing a site-specific temperature-sensitive and attenuating mutation.

Authors:  E K Subbarao; Y Kawaoka; B R Murphy
Journal:  J Virol       Date:  1993-12       Impact factor: 5.103

6.  A novel universal primer-multiplex-PCR method with sequencing gel electrophoresis analysis.

Authors:  Wentao Xu; Zhifang Zhai; Kunlun Huang; Nan Zhang; Yanfang Yuan; Ying Shang; Yunbo Luo
Journal:  PLoS One       Date:  2012-01-17       Impact factor: 3.240

7.  Silver and Cyanine Staining of Oligonucleotides in Polyacrylamide Gel.

Authors:  Weizhong Tang; Huafu Zhou; Wei Li
Journal:  PLoS One       Date:  2015-12-09       Impact factor: 3.240
  7 in total

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