Literature DB >> 6171307

Monoclonal antibodies against human acid alpha-glucosidase.

J Hilkens, J M Tager, F Buijs, B Brouwer-Kelder, G M Van Thienen, F P Tegelaers, J Hilgers.   

Abstract

Acid alpha-glucosidase purified from human placenta was used to immunize a mouse (strain Balb/cHeA) according to a procedure described earlier (Stähli, C., Staehlin, T., Miggiano, V., Schmidt, J. and Häring, P. (1980) J. Immunol. Methods 32, 297-304). After fusion of spleen cells with myeloma cells, about 10% of the hybrid clones obtained produced antibodies against acid alpha-glucosidase. Finally, eight stable clones producing antibodies against the enzyme were obtained. When purified acid alpha-glucosidase is analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, two major protein bands (mol. wt. 76000 and 70000,) a minor band of mol. wt. 9600 and several minor bands with a mol. wt. of 67000 or lower are seen. Since all these components react with the monoclonal antibodies, they must have at least one antigenic determinant in common.

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Year:  1981        PMID： 6171307     DOI： 10.1016/0304-4165(81)90041-6

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  7 in total

1.  Identification of a missense mutation in one allele of a patient with Pompe disease, and use of endonuclease digestion of PCR-amplified RNA to demonstrate lack of mRNA expression from the second allele.

Authors:  N Zhong; F Martiniuk; S Tzall; R Hirschhorn
Journal:  Am J Hum Genet       Date:  1991-09       Impact factor: 11.025

2.  Heterogeneity of pig lysosomal acid alpha-glucosidase. Affinity to Sephacryl S-200 gel and tissue distribution.

Authors:  S Nakasone; T Ohshita; T Iwamasa
Journal:  Biochem J       Date:  1991-11-01       Impact factor: 3.857

3.  Mutations of glucocerebrosidase: discrimination of neurologic and non-neurologic phenotypes of Gaucher disease.

Authors:  E I Ginns; R O Brady; S Pirruccello; C Moore; S Sorrell; F S Furbish; G J Murray; J Tager; J A Barranger
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

4.  Assignment of the gene coding for human beta-glucocerebrosidase to the region q21-q31 of chromosome 1 using monoclonal antibodies.

Authors:  R A Barneveld; W Keijzer; F P Tegelaers; E I Ginns; A Geurts van Kessel; R O Brady; J A Barranger; J M Tager; H Galjaard; A Westerveld; A J Reuser
Journal:  Hum Genet       Date:  1983       Impact factor: 4.132

5.  Immunocytochemistry of lysosomal hydrolases and their precursor forms in normal and mutant human cells.

Authors:  J M Van Dongen; R A Barneveld; H J Geuze; H Galjaard
Journal:  Histochem J       Date:  1984-09

6.  The immunohistochemical reactivity of a new anti-epithelial monoclonal antibody (MAb b-12) against breast carcinoma and other normal and neoplastic human tissues.

Authors:  H R Zenklusen; C Stähli; F Gudat; J von Overbeck; J Rolink; P U Heitz
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1988

7.  Nocodazole, a microtubule-active drug, interferes with apical protein delivery in cultured intestinal epithelial cells (Caco-2).

Authors:  U Eilers; J Klumperman; H P Hauri
Journal:  J Cell Biol       Date:  1989-01       Impact factor: 10.539
  7 in total

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