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Literature DB >> 6171208

Changes in suspensions of human keratinocytes due to trypsin.

Abstract

Epidermal cell suspensions derived from keratotomed samples of normal human skin have been studied by light microscopy and by scanning and transmission electron microscope techniques. The method allows detailed inspection of the keratinocyte membranes. However, the trypsin used to obtain the suspensions limits the usefulness of the technique as some of the features observed - including invagination of desmosomes, vacuolation, and redistribution of tonofibrils - may be attributable to this proteolytic enzyme.

Entities: Species

Mesh：

Substances：
Keratins
Trypsin

Year: 1981 PMID： 6171208 DOI： 10.1007/BF00409453

Source DB: PubMed Journal: Arch Dermatol Res ISSN： 0340-3696 Impact factor: 3.017

11 in total

1. Ultrastructural studies of newborn rat epidermis after trypsinization.

Authors: K Fukuyama; M M Black; W L Epstein
Journal: J Ultrastruct Res Date: 1974-02

2. Scanning electron microscopy of cells in culture.

Authors: A Boyde; R A Weiss; P Veselý
Journal: Exp Cell Res Date: 1972 Impact factor: 3.905

3. Intraepidermal cell surface fine structure: preservation and examination at high resolution.

Authors: T E Lempert; P M Elias
Journal: Anat Rec Date: 1979-04

4. A technique for studying the structural detail of isolated human corneocytes.

Authors: S P Barton; C S King; R Marks; S Nicholls
Journal: Br J Dermatol Date: 1980-01 Impact factor: 9.302

5. The experimental production of high-level intraepidermal splits.

Authors: C J Skerrow
Journal: Br J Dermatol Date: 1980-01 Impact factor: 9.302

6. Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization.

Authors: J M Gommans; M Bergers; P E van Erp; J J van den Hurk; P D Mier; H Roelfzema
Journal: Br J Dermatol Date: 1979-10 Impact factor: 9.302

7. Lipid compositions of cells isolated from pig, human, and rat epidermis.

Authors: G M Gray; H J Yardley
Journal: J Lipid Res Date: 1975-11 Impact factor: 5.922

8. Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.

Authors: K G Vogel
Journal: Exp Cell Res Date: 1978-05 Impact factor: 3.905

4. α6 Integrin (α6^high)/Transferrin Receptor (CD71)^low Keratinocyte Stem Cells Are More Potent for Generating Reconstructed Skin Epidermis Than Rapid Adherent Cells.

Authors: Elodie Metral; Nicolas Bechetoille; Frédéric Demarne; Walid Rachidi; Odile Damour
Journal: Int J Mol Sci Date: 2017-01-27 Impact factor: 5.923

4 in total

Changes in suspensions of human keratinocytes due to trypsin.

1. Ultrastructural studies of newborn rat epidermis after trypsinization.

2. Scanning electron microscopy of cells in culture.

3. Intraepidermal cell surface fine structure: preservation and examination at high resolution.

4. A technique for studying the structural detail of isolated human corneocytes.

5. The experimental production of high-level intraepidermal splits.

6. Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization.

7. Lipid compositions of cells isolated from pig, human, and rat epidermis.

8. Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.

9. Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization.

10. The uptake of 14C-leucine and 14C-histidine by cell suspensions of isolated strata of neonatal rat epidermis.

1. Alterations in membrane fluidity during keratinocyte differentiation measured by fluorescence polarization.

2. Fractionation of Paget cells in extramammary Paget's disease.

Review 3. Pharmacotherapy of acute lung injury and the acute respiratory distress syndrome.

4. α6 Integrin (α6^high)/Transferrin Receptor (CD71)^low Keratinocyte Stem Cells Are More Potent for Generating Reconstructed Skin Epidermis Than Rapid Adherent Cells.