Lymphocyte-dependent antibodies to antigen 3.1, a cell-surface antigen expressed by a subgroup of human melanomas.

Previous work from our laboratory has led to the isolation of a monoclonal IgG2a mouse antibody, 3.2, which is specific for an antigen, 3.1, that is strongest expressed by a group of human melanomas. We have no demonstrated that this antibody can mediate antibody-dependent cellular cytotoxicity (ADCC) to human melanoma cells expressing antigen 3., and that lymphocytes from human peripheral blood can be used as the source of K cells. A monoclonal IgG1 antibody, 3.1, to the same determinant of antigen 3.1 gave only weak and inconsistent ADCC.