Literature DB >> 6169493

Simplified method for DNA and protein staining of human hematopoietic cell samples.

H A Crissman, J Van Egmond, R S Holdrinet, A Pennings, C Haanen.   

Abstract

A rapid reproducible method yielding high resolution analysis of DNA and protein in human hematopoietic cell samples has been developed by modification of the propidium iodide and fluorescein isothiocyanate procedure. Cell staining involves sequential addition of each reagent (RNase, fluorescein isothiocyanate and propidium iodide) to ethanol-fixed cells and requires no centrifugation steps. Stained cells are analyzed in the reagent solutions. Analysis of bone marrow samples from multiple myeloma patients showed mixed normal and aneuploid populations with a major portion of the aneuploid cells having a significantly higher protein content. This approach permitted differential cell cycle analysis of normal and the aneuploid populations.

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Year:  1981        PMID： 6169493     DOI： 10.1002/cyto.990020204

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  7 in total

1.  Functional heterogeneity of human antigen-presenting cells: presentation of soluble antigen but not self-Ia by monocytes.

Authors:  J Moreno; P E Lipsky
Journal:  J Clin Immunol       Date:  1986-01       Impact factor: 8.317

2.  Non-steroidal anti-inflammatory drugs and prostaglandin effects on pepsinogen secretion by dispersed human peptic cells.

Authors:  A I Lanas; J Nerín; F Esteva; R Sáinz
Journal:  Gut       Date:  1995-05       Impact factor: 23.059

3.  Fructose-induced enhanced mitogenicity of diploid human cells: possible relationship with cell differentiation.

Authors:  C Wolfrom; N Kadhom; J Raulin; N Raynaud; M Gautier
Journal:  In Vitro Cell Dev Biol Anim       Date:  1994-04       Impact factor: 2.416

4.  Studies on urinary bladder carcinoma by morphometry, flow cytometry, and light microscopic malignancy grading with special reference to grade II tumours.

Authors:  K Helander; B Kirkhus; O H Iversen; S L Johansson; S Nilsson; S Vaage; H Fjordvang
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1985

5.  Choice of fixation and denaturation for the triple labelling of intra-cytoplasmic antigen, bromodeoxyuridine and DNA. Application to bone marrow plasma cells.

Authors:  M Ffrench; F Morel; C Souchier; M Benchaib; R Catallo; P A Bryon
Journal:  Histochemistry       Date:  1994-06

6.  Induction of cell cycle regulatory proteins in anti-immunoglobulin-stimulated mature B lymphocytes.

Authors:  N Solvason; W W Wu; N Kabra; X Wu; E Lees; M C Howard
Journal:  J Exp Med       Date:  1996-08-01       Impact factor: 14.307

7.  Normal and perturbed Chinese hamster ovary cells: correlation of DNA, RNA, and protein content by flow cytometry.

Authors:  H A Crissman; Z Darzynkiewicz; R A Tobey; J A Steinkamp
Journal:  J Cell Biol       Date:  1985-07       Impact factor: 10.539
  7 in total

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