| Literature DB >> 6168644 |
M Golomb, A C Vora, D P Grandgenett.
Abstract
Reverse transcriptase from Rous sarcoma virus and avian myeloblastosis virus was purified by a rapid two-step procedure using chromatography on phosphocellulose and heparin-Sepharose. The resulting enzyme was homogeneous, had a high specific activity and was free of contaminating nucleases. This procedure has been adapted to small-scale preparation of enzyme from mutant virus containing thermolabile reverse transcriptase, and is equally suitable for large-scale enzyme purification.Entities:
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Year: 1980 PMID： 6168644 DOI： 10.1016/0166-0934(80)90012-9
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014