| Literature DB >> 6168455 |
P D Noguchi, J B Johnson, W Browne.
Abstract
Cells were stained for DNA content with Hoechst 33258 combined with propidium iodide and analyzed on an epi-fluorescence flow cytometer. Scatterplots of red versus blue fluorescence of control cells showed a straight line indicating that both dyes were measuring proportional amounts of cellular DNA. Scatterplots of cells incubated with the thymidine analog bromodeoxyuridine, however, showed a displacement from the control straight line that increased with longer exposure time to bromodeoxyuridine. These results suggest that bromodeoxyuridine incorporation in DNA can be detected by flow cytometry.Entities:
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Year: 1981 PMID： 6168455 DOI： 10.1002/cyto.990010607
Source DB: PubMed Journal: Cytometry ISSN： 0196-4763