Literature DB >> 6168453

Flow cytometry of human reticulocytes based on RNA fluorescence.

H J Tanke, I A Nieuwenhuis, G J Koper, J C Slats, J S Ploem.   

Abstract

A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting. Histograms of fluorescence values of mature red blood cells and reticulocytes obtained by flow cytometry of stained blood samples, contain more information than offered by conventional methods for counting reticulocytes. Analysis of the fluorescence histograms provides new descriptive parameters of the reticulocyte populations. The potential importance of this method in experimental and routine hematology is illustrated for a number of clinical cases.

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Year:  1981        PMID： 6168453     DOI： 10.1002/cyto.990010503

Source DB:  PubMed          Journal:  Cytometry        ISSN： 0196-4763


  6 in total

Review 1.  Reticulocytes and reticulocyte enumeration.

Authors:  R S Riley; J M Ben-Ezra; R Goel; A Tidwell
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

2.  [Automated microfluorometric absolute count and maturation analysis of reticulocytes].

Authors:  H Eder; H Fritsche
Journal:  Klin Wochenschr       Date:  1989-10-17

3.  Changes in erythropoiesis due to radiation or chemotherapy as studied by flow cytometric determination of peripheral blood reticulocytes.

Authors:  H J Tanke; P H van Vianen; F M Emiliani; I Neuteboom; N de Vogel; A D Tates; E A de Bruijn; A T van Oosterom
Journal:  Histochemistry       Date:  1986

4.  Supravital experiments with Pyronin Y, a fluorochrome of mitochondria and nucleic acids.

Authors:  R R Cowden; S K Curtis
Journal:  Histochemistry       Date:  1983

5.  Flow-cytometric enumeration of reticulocytes with the new fluorochrome 1,3'-diethyl-4,2'-quinolylthiacyanine (correction of 1',3'-diethyl-4,2'-quinolylthiacyanine)

Authors:  R Guasch; G Juan; F Carretero; J E O'Connor
Journal:  Ann Hematol       Date:  1992-10       Impact factor: 3.673

6.  Normal and perturbed Chinese hamster ovary cells: correlation of DNA, RNA, and protein content by flow cytometry.

Authors:  H A Crissman; Z Darzynkiewicz; R A Tobey; J A Steinkamp
Journal:  J Cell Biol       Date:  1985-07       Impact factor: 10.539
  6 in total

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