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Literature DB >> 6167641

A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation.

Abstract

A simple one-step method for purification of human granulocytes, based on the use of a discontinuous gradient of Percoll, is described. The cell yield is 55% and the purity of the granulocyte fraction is 97%. The cells' ability to exclude trypan blue and phagocytic function are not altered during the purification process. We conclude that centrifugation in Percoll is superior to conventional dextran sedimentation; it is also less time-consuming.

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Year: 1981 PMID： 6167641 DOI： 10.1016/0022-1759(81)90040-5

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

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Cited

51 in total

1. Calcium signalling is altered in myeloid cells with a deficiency in NADPH oxidase activity.

Authors: B K Rada; M Geiszt; R Van Bruggen; K Nemet; D Roos; E Ligeti
Journal: Clin Exp Immunol Date: 2003-04 Impact factor: 4.330

2. Myeloperoxidase of polymorphonuclear leukocytes in psoriasis vulgaris.

Authors: H Greiten; M Rister; G K Steigleder
Journal: Arch Dermatol Res Date: 1987 Impact factor: 3.017

3. [Measuring chemiluminescence in phagocytic granulocytes--simultaneously a parameter of their killing function?].

Authors: L Dziwisch; B Kremer; J Heesemann; D Bornholdt; D Henne-Bruns
Journal: Langenbecks Arch Chir Date: 1987

4. Inhibition of granulocyte function by steroids is not limited to corticoids. Studies with sex steroids.

Authors: D E Hammerschmidt; A C Knabe; P T Silberstein; H R Lamche; P A Coppo
Journal: Inflammation Date: 1988-06 Impact factor: 4.092

5. Parallel determination of glucocorticoid receptors in human mononuclear and polymorphonuclear leukocytes after Percoll separation.

Authors: D Armanini; T Strasser; P C Weber
Journal: J Endocrinol Invest Date: 1985-02 Impact factor: 4.256

A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation.

1. Calcium signalling is altered in myeloid cells with a deficiency in NADPH oxidase activity.

2. Myeloperoxidase of polymorphonuclear leukocytes in psoriasis vulgaris.

3. [Measuring chemiluminescence in phagocytic granulocytes--simultaneously a parameter of their killing function?].

4. Inhibition of granulocyte function by steroids is not limited to corticoids. Studies with sex steroids.

5. Parallel determination of glucocorticoid receptors in human mononuclear and polymorphonuclear leukocytes after Percoll separation.

6. Persistent bactericidal defect in neutrophils from a young woman who recovered from toxic shock syndrome.

7. Effects of aminoglycoside antibiotics on polymorphonuclear leukocyte function in vivo.

8. Pathogenesis of foreign body infection. Evidence for a local granulocyte defect.

9. The influence of intravenous anaesthetics on polymorphonuclear leukocyte function.

10. Lineage-specific and differentiation-stage-specific gene expression in normal and leukaemic human myeloid cells.