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Literature DB >> 6164428

The effects of nonidet P40 on the function of rat peritoneal mast cells in vitro.

Abstract

1 Treatment of purified rat peritoneal mast cells at 37 degrees C with concentrations of the non-ionic detergent nonidet P40 (NP40) up to 0.005% (v/v) failed to reduce their viability. 2 There was a marked reduction in the histamine releasing capacity of NP40-treated mast cells upon challenge with a variety of selective (adrenocorticotrophic hormone 1-24 (Synacthen), rabbit anti-rat IgE antiserum, adenosine triphosphate (ATP) and the calcium ionophore, A 23187) and non-selective (rabbit anti-rat mast cell antiserum plus complement) histamine liberators. 3 Nonidet P40 (0.005%) was found to reduce the activity of a mast cell membrane 'ecto-enzyme', calcium-activated ATPase, by about 45% when presented at the time of its assay.

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Year: 1981 PMID： 6164428 PMCID： PMC2071542 DOI： 10.1111/j.1476-5381.1981.tb09110.x

Source DB: PubMed Journal: Br J Pharmacol ISSN： 0007-1188 Impact factor: 8.739

8 in total

6. Plasma-membrane components can be removed from isolated lymphocytes by the bile salts glycocholate and taurocholate without cell lysis.

Authors: G Holdsworth; R Coleman
Journal: Biochem J Date: 1976-08-15 Impact factor: 3.857

7. Effect of removal of calcium-activated adenosine triphosphatase from rat mast cells by treatment with sodium glycocholate.

Authors: P H Cooper; D R Stanworth
Journal: Biochem J Date: 1977-12-15 Impact factor: 3.857

8. Characterization of calcium-ion-activated adenosine triphosphatase in the plasma membrane of rat mast cells.

Authors: P H Cooper; D R Stanworth
Journal: Biochem J Date: 1976-06-15 Impact factor: 3.857

8 in total

2 in total

1. Mechanism of histamine release from rat peritoneal mast cells.

Authors: S A Patkar; B Diamant
Journal: Klin Wochenschr Date: 1982-09-01

2. Histamine release and hypotensive reactions in dogs by solubilizing agents and fatty acids: analysis of various components in cremophor El and development of a compound with reduced toxicity.

Authors: W Lorenz; A Schmal; H Schult; S Lang; C Ohmann; D Weber; B Kapp; L Lüben; A Doenicke
Journal: Agents Actions Date: 1982-04

2 in total

The effects of nonidet P40 on the function of rat peritoneal mast cells in vitro.

1. Non-lytic, non-ionic detergent extraction of plasma membrane constituents from normal and transformed fibroblasts.

2. The release of membrane components prior to haemolysis during extraction of intact eryghrocytes with bile salts.

3. A simple and reproducible method of isolating rat peritoneal mast cells in high yield and purity.

4. An automated fluorimetric assay for the rapid determination of histamine in biological fluids.

5. Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions.

6. Plasma-membrane components can be removed from isolated lymphocytes by the bile salts glycocholate and taurocholate without cell lysis.

7. Effect of removal of calcium-activated adenosine triphosphatase from rat mast cells by treatment with sodium glycocholate.

8. Characterization of calcium-ion-activated adenosine triphosphatase in the plasma membrane of rat mast cells.

1. Mechanism of histamine release from rat peritoneal mast cells.

2. Histamine release and hypotensive reactions in dogs by solubilizing agents and fatty acids: analysis of various components in cremophor El and development of a compound with reduced toxicity.