Literature DB >> 6161832

A new approach to the morphological and cytochemical evaluation of human bone marrow CFUC in agar cultures.

G Konwalinka, P Glaser, R Odavic, E Bogusch, F Schmalzl, H Braunsteiner.   

Abstract

A simple procedure is described for the routine morphological examination of different types of colonies and clusters in agar cultures. The following cytochemical methods are used for the identification of cells: naphthol-ASD-chloroacetate esterase, naphthol-ASD-acetate esterase with and without addition of sodium fluoride, Biebrich scarlet and acid phosphatase. Based on their cytochemical staining properties neutrophils, eosinophils, monocytes and macrophages can be readily identified in their different stages of maturation and an assessment made of the cellular composition of colonies and clusters.

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Year:  1980        PMID： 6161832

Source DB:  PubMed          Journal:  Exp Hematol        ISSN： 0301-472X            Impact factor:   3.084


  7 in total

1.  Simple method for granulocyte-macrophage cell culture and staining in soft agar: comparison with a standard research technique.

Authors:  K Hyde; A J Steed; H Lenehan; M J Saunders; J T Richards; J A Lin Yin; C G Geary
Journal:  J Clin Pathol       Date:  1989-12       Impact factor: 3.411

2.  Establishment and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells.

Authors:  A Pessina; E Mineo; M G Neri; L Gribaldo; R Colombi; P Brambilla; G Zaleskis
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

3.  Immunocytochemical identification of murine and human megakaryocyte colonies in soft-agar cultures.

Authors:  J L Zhang; P E Stenberg; G Baker; J Levin
Journal:  Histochem J       Date:  1994-02

4.  Methods for evaluating the morphological and immunohistochemical properties of human tumor colonies grown in soft agar.

Authors:  B Persky; S P Thomson; F L Meyskens; M J Hendrix
Journal:  In Vitro       Date:  1982-11

5.  The role of B-cells in immunity against adult Strongyloides venezuelensis.

Authors:  Mohamed A El-Malky; Haruhiko Maruyama; Saeed A Al-Harthi; Samar N El-Beshbishi; Nobu Ohta
Journal:  Parasit Vectors       Date:  2013-05-24       Impact factor: 3.876

6.  Purification and characterization of human granulocyte colony-stimulating factor (G-CSF).

Authors:  H Nomura; I Imazeki; M Oheda; N Kubota; M Tamura; M Ono; Y Ueyama; S Asano
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598

7.  A recombinant murine granulocyte/macrophage (GM) colony-stimulating factor derived from an inducer T cell line (IH5.5). Functional restriction to GM progenitor cells.

Authors:  S Kajigaya; T Suda; J Suda; M Saito; Y Miura; M Iizuka; S Kobayashi; N Minato; T Sudo
Journal:  J Exp Med       Date:  1986-10-01       Impact factor: 14.307
  7 in total

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