Literature DB >> 6160852

Preparation of resealed pig lymphocyte plasma-membrane vesicles.

A P Johnstone, M J Crumpton.   

Abstract

Pig lymphocyte plasma-membrane vesicles that are impermeable to large molecules were prepared by means of centrifugation on a continuous dextran gradient. They were isolated in a yield of 60--80% of the total plasma membrane recovered, and were characterized as being impermeable to macromolecules by their exclusion of inulin (mol.wt. 5200) and Dextran T10 (mol.wt. 10 000).

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Year:  1980        PMID: 6160852      PMCID: PMC1162062          DOI: 10.1042/bj1900045

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  12 in total

Review 1.  The structure and metabolism of lymphocyte membranes.

Authors:  C T Ladoulis; T J Gill; S H Chen; D N Misra
Journal:  Prog Allergy       Date:  1975

2.  Orientation of cell-surface antigens in the lipid bilayer of lymphocyte plasma membrane.

Authors:  F S Walsh; M J Crumpton
Journal:  Nature       Date:  1977-09-22       Impact factor: 49.962

3.  Disruption of solid tissue for plasma membrane preparation.

Authors:  D Snary; F R Woods; M J Crumpton
Journal:  Anal Biochem       Date:  1976-08       Impact factor: 3.365

4.  Use of a concanavalin A polymer to isolate right side-out vesicles of purified plasma membranes from eukariotic cells.

Authors:  A Zachowski; A Paraf
Journal:  Biochem Biophys Res Commun       Date:  1974-04-08       Impact factor: 3.575

5.  Preparation and characterization of the plasma membrane of pig lymphocytes.

Authors:  D Allan; M J Crumpton
Journal:  Biochem J       Date:  1970-11       Impact factor: 3.857

6.  Preparation of inverted plasma membrane vesicles from isolated adipocytes.

Authors:  V Bennett; P Cuatrecasas
Journal:  Biochim Biophys Acta       Date:  1973-07-06

7.  Topographical distribution of complex carbohydrates in the erythrocyte membrane.

Authors:  T L Steck; G Dawson
Journal:  J Biol Chem       Date:  1974-04-10       Impact factor: 5.157

8.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

9.  Preparation of inside-out vesicles of pig lymphocyte plasma membrane.

Authors:  F S Walsh; B H Barber; M J Crumpton
Journal:  Biochemistry       Date:  1976-08-10       Impact factor: 3.162

10.  Active amino acid transport in plasma membrane vesicles from Simian virus 40-transformed mouse fibroblasts. Characteristics of electrochemical Na+ gradient-stimulated uptake.

Authors:  J E Lever
Journal:  J Biol Chem       Date:  1977-03-25       Impact factor: 5.157

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  2 in total

1.  The use of horseradish peroxidase-lectin conjugates to monitor the purity of plasma-membrane preparations obtained from isolated cells.

Authors:  J P Banga; P Penfold; I M Roitt
Journal:  Biochem J       Date:  1981-08-15       Impact factor: 3.857

2.  Rapid preparation of plasma membranes from avian lymphoid cells and fibroblasts for virus binding studies.

Authors:  H Nieper; H Müller
Journal:  J Virol Methods       Date:  1998-06       Impact factor: 2.014

  2 in total

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