| Literature DB >> 6160430 |
H Akagi, M Otsuka, M Yanagisawa.
Abstract
An attempt was made to identify the immunoreactive substance P (SP) released from isolated rat spinal cords using reversed-phase high-performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA) for SP. Soaking the spinal cords of newborn rats in Krebs solution containing 90 mM K+ evoked a release of immunoreactive SP as well as of GABA and glycine in a calcium-dependent manner. Capsaicin also evoked a release of immunoreactive SP but not of GABA and glycine. The immunoreactive SP released from rat spinal cords by high K+ or capsaicin was analyzed by HPLC. A single peak was detected by RIA whose elution position coincided with that of synthetic SP.Entities:
Mesh：
Substances：
Year: 1980 PMID： 6160430 DOI： 10.1016/0304-3940(80)90157-3
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046