Literature DB >> 6159630

Identification of folate binding protein of mitochondria as dimethylglycine dehydrogenase.

A J Wittwer, C Wagner.   

Abstract

The folate-binding protein of rat liver mitochondria [Zamierowski, M. & Wagner, C. (1977) J. Biol. Chem. 252, 933-938] has been purified to homogeneity by a combination of gel filtration, DEAE-cellulose, and affinity chromatography. This protein was assayed by its ability to bind tetrahydro[3H]folic acid in vitro. the purified protein contains tightly bound flavin and has a molecular weight of about 90,000 as determined by sodium dodecyl sulfate electrophoresis. This protein also displays dimethylglycine dehydrogenase [N,N-dimethylglycine: (acceptor) oxidoreductase (demethylating), EC 1.5.99.2] activity which copurifies with the folate-binding activity. It is suggested that the role of the tetrahydrofolic acid is to accept the formaldehyde produced during the course of the reaction.

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Year:  1980        PMID： 6159630      PMCID： PMC349868          DOI： 10.1073/pnas.77.8.4484

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

1.  Oxidation of one-carbon compounds to formate and carbon dioxide in rat liver mitochondria.

Authors:  K F Lewis; V M Randolph; E Nemeth; W R Frisell
Journal:  Arch Biochem Biophys       Date:  1978-01-30       Impact factor: 4.013

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Proteins with covalently-bound flavin in rat liver mitochondria.

Authors:  M Sato; N Ohishi; M Nishikimi; K Yagi
Journal:  Biochem Biophys Res Commun       Date:  1977-10-10       Impact factor: 3.575

4.  The purification and characterization of the low molecular weight human folate binding protein using affinity chromatography.

Authors:  S Waxman; C Schreiber
Journal:  Biochemistry       Date:  1975-12-16       Impact factor: 3.162

5.  A rapid method for desalting small volumes of solution.

Authors:  M W Neal; J R Florini
Journal:  Anal Biochem       Date:  1973-09       Impact factor: 3.365

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Biogenesis of flavoprotein and cytochrome components in hepatic mitochondria from riboflavin-deficient rats.

Authors:  R Addison; D B McCormick
Journal:  Biochem Biophys Res Commun       Date:  1978-03-15       Impact factor: 3.575

8.  Reversible binding of Pi by beef heart mitochondrial adenosine triphosphatase.

Authors:  H S Penefsky
Journal:  J Biol Chem       Date:  1977-05-10       Impact factor: 5.157

9.  Identification of folate binding proteins in rat liver.

Authors:  M M Zamierowski; C Wagner
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

10.  A convenient method for the preparation of dl-5-methyltetrahydrofolic acid (dl-5-methyl-5,6,7,8-tetrahydropteroyl-L-monoglutamic acid).

Authors:  J A Blair; K J Saunders
Journal:  Anal Biochem       Date:  1970-04       Impact factor: 3.365
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  15 in total

1.  Histone demethylase LSD1 is a folate-binding protein.

Authors:  Zigmund Luka; Frank Moss; Lioudmila V Loukachevitch; Darryl J Bornhop; Conrad Wagner
Journal:  Biochemistry       Date:  2011-05-05       Impact factor: 3.162

2.  Tissue specificity of rat mitochondrial dimethylglycine dehydrogenase expression.

Authors:  H Lang; K Minaian; N Freudenberg; R Hoffmann; R Brandsch
Journal:  Biochem J       Date:  1994-04-15       Impact factor: 3.857

3.  Cloning of dimethylglycine dehydrogenase and a new human inborn error of metabolism, dimethylglycine dehydrogenase deficiency.

Authors:  B A Binzak; R A Wevers; S H Moolenaar; Y M Lee; W L Hwu; J Poggi-Bach; U F Engelke; H M Hoard; J G Vockley; J Vockley
Journal:  Am J Hum Genet       Date:  2001-02-28       Impact factor: 11.025

4.  Mutations in the sarcosine dehydrogenase gene in patients with sarcosinemia.

Authors:  Ifat Bar-joseph; Elon Pras; Haike Reznik-Wolf; Dina Marek-Yagel; Almogit Abu-Horvitz; Maya Dushnitzky; Nurit Goldstein; Shlomit Rienstein; Michal Dekel; Ben Pode-Shakked; Joseph Zlotnik; Anelia Benarrosh; Philippe Gillery; Niklaus Hofliger; Christiane Auray-Blais; Roselyne Garnotel; Yair Anikster
Journal:  Hum Genet       Date:  2012-07-24       Impact factor: 4.132

5.  A single arginine residue is required for the interaction of the electron transferring flavoprotein (ETF) with three of its dehydrogenase partners.

Authors:  Antony R Parker
Journal:  Mol Cell Biochem       Date:  2003-12       Impact factor: 3.396

6.  Glycine N-methyltransferase is a folate binding protein of rat liver cytosol.

Authors:  R J Cook; C Wagner
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

7.  Molecular basis of dimethylglycine dehydrogenase deficiency associated with pathogenic variant H109R.

Authors:  R P McAndrew; J Vockley; J-J P Kim
Journal:  J Inherit Metab Dis       Date:  2008-10-21       Impact factor: 4.982

8.  The effect of tetrahydrofolate on the reduction of electron transfer flavoprotein by sarcosine and dimethylglycine dehydrogenases.

Authors:  D J Steenkamp; M Husain
Journal:  Biochem J       Date:  1982-06-01       Impact factor: 3.857

9.  Partial purification and characterization of glutaryl-coenzyme A dehydrogenase, electron transfer flavoprotein, and electron transfer flavoprotein-Q oxidoreductase from Paracoccus denitrificans.

Authors:  M Husain; D J Steenkamp
Journal:  J Bacteriol       Date:  1985-08       Impact factor: 3.490

Review 10.  Enzymatic synthesis and function of folylpolyglutamates.

Authors:  J J McGuire; J R Bertino
Journal:  Mol Cell Biochem       Date:  1981-08-11       Impact factor: 3.396
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