Purification of canine myocardial mitochondrial creatine kinase.

Mitochondrial creatine kinase, purified for the first time, is a dimeric molecule with a mol. wt of 82,000 and does not hybridize with M or B subunits or react to their specific antiserum. A specific antiserum to the mitochondrial form was developed which does not cross-react with the B or M subunits. Thus, the mitochondrial form is biochemically and immunologically unique.