Literature DB >> 6157671

Kinetics and regulation of cell-free alkaline phosphatase synthesis.

C Pratt.   

Abstract

Regulation of alkaline phosphatase (EC 3.1.3.1) synthesis in a cell-free system from Escherichia coli has been observed. Synthesis from transducing phage deoxyribonucleic acid templates carrying phoA+ occurred in S30 fractions from wild-type or alkaline phosphatase-constitutive mutants. It did not occur in S30) fractions from alkaline phosphatase-negative mutants (phoB). The hybrid gene phoA-lacZ was also subject to phoB control, implying that phoA transcription is regulated. The yield of active alkaline phosphatase per phoA+ gene copy from cell-free synthesis was similar to that of beta-galactosidase. Alkaline phosphatase activity took longer to appear than beta-galactosidase activity. Synthesis of alkaline phosphatase subunits was not delayed, suggesting that a minimum number of subunits are synthesized before formation of active alkaline phosphatase occurs.

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Year:  1980        PMID： 6157671      PMCID： PMC294493          DOI： 10.1128/jb.143.3.1265-1274.1980

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  42 in total

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Authors:  W Wetekam; K Staack; R Ehring
Journal:  Mol Gen Genet       Date:  1971

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Authors:  M Jacquet; A Kepes
Journal:  Biochem Biophys Res Commun       Date:  1969-07-07       Impact factor: 3.575

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Journal:  Biochem Biophys Res Commun       Date:  1969-02-21       Impact factor: 3.575

4.  Purification and properties of intracellular lamba DNA rings.

Authors:  J A Kiger; E T Young; R L Sinsheimer
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  Mode of action of rafamycin on the RNA polymerase reaction.

Authors:  A Sippel; G Hartmann
Journal:  Biochim Biophys Acta       Date:  1968-03-18

6.  The reversible dissociation of the alkaline phosphatase of Escherichia coli. I. Formation and reactivation of subunits.

Authors:  M J Schlesinger; K Barrett
Journal:  J Biol Chem       Date:  1965-11       Impact factor: 5.157

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Physiological factors in the regulation of alkaline phosphatase synthesis in Escherichia coli.

Authors:  A S Wilkins
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

9.  Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation.

Authors:  G R Willsky; R L Bennett; M H Malamy
Journal:  J Bacteriol       Date:  1973-02       Impact factor: 3.490

10.  Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli.

Authors:  G Zubay; D E Morse; W J Schrenk; J H Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1972-05       Impact factor: 11.205
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  7 in total

1.  Cloning of immunity and structural genes for colicin V.

Authors:  K K Frick; R L Quackenbush; J Konisky
Journal:  J Bacteriol       Date:  1981-11       Impact factor: 3.490

2.  Cloning and characterization of the Escherichia coli gene coding for alkaline phosphatase.

Authors:  P E Berg
Journal:  J Bacteriol       Date:  1981-05       Impact factor: 3.490

3.  DNA-directed in vitro synthesis and assembly of the form II D-ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodopseudomonas sphaeroides.

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Journal:  J Bacteriol       Date:  1985-01       Impact factor: 3.490

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Authors:  N N Rao; E Wang; J Yashphe; A Torriani
Journal:  J Bacteriol       Date:  1986-04       Impact factor: 3.490

5.  Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies.

Authors:  J A Kanabrocki; R L Quackenbush; F R Pond
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

6.  Cloning and expression of the fbc operon encoding the FeS protein, cytochrome b and cytochrome c1 from the Rhodopseudomonas sphaeroides b/c1 complex.

Authors:  N Gabellini; U Harnisch; J E McCarthy; G Hauska; W Sebald
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598

7.  Translational initiation frequency of atp genes from Escherichia coli: identification of an intercistronic sequence that enhances translation.

Authors:  J E McCarthy; H U Schairer; W Sebald
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
  7 in total

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