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Literature DB >> 6157387

Specific quantification of mouse and human keratin proteins by radioimmunoassay.

S H Yuspa, M C Poirier, J R Harness, D R Olsom, P M Steinert.

Abstract

Mouse epidermal keratin proteins were purified and labelled with 125I by chemical techniques. A radioimmunoassay method was established with a rabbit antibody elicited against the mouse keratin. This assay method was utilized to quantify keratin proteins of mouse and human epidermal extracts, both from intact tissues and cells in culture. As little as 30 ng of mouse keratin (0.5 pmol) was quantifiable.

Entities: Species

Mesh：

Substances：
Keratins

Year: 1980 PMID： 6157387 PMCID： PMC1162523 DOI： 10.1042/bj1870281

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

20 in total

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors: F C GREENWOOD; W M HUNTER; J S GLOVER
Journal: Biochem J Date: 1963-10 Impact factor: 3.857

2. Self-assembly of bovine epidermal keratin filaments in vitro.

Authors: P M Steinert; W W Idler; S B Zimmerman
Journal: J Mol Biol Date: 1976-12-15 Impact factor: 5.469

3. Hormonal regulation of epidermis-specific protein and messenger RNA synthesis in amphibian metamorphosis.

Authors: R Reeves
Journal: Dev Biol Date: 1977-10-01 Impact factor: 3.582

4. Altered differentiation of mouse epidermal cells treated with retinyl acetate in vitro.

Authors: S H Yuspa; C C Harris
Journal: Exp Cell Res Date: 1974-05 Impact factor: 3.905

5. A simple colorimetric method for determination of protein.

Authors: S Bramhall; N Noack; M Wu; J R Loewenberg
Journal: Anal Biochem Date: 1969-10-01 Impact factor: 3.365

6. Immunology of epidermal fibrous proteins.

Authors: L D Lee; H P Baden; J Kunilus; B F Fleming
Journal: J Invest Dermatol Date: 1976-10 Impact factor: 8.551

7. Adult amphibian epidermal proteins: biochemical characterization and developmental appearance.

Authors: O R Reeves
Journal: J Embryol Exp Morphol Date: 1975-08

8. The polypeptide composition of bovine epidermal alpha-keratin.

Authors: P M Steinert; W W Idler
Journal: Biochem J Date: 1975-12 Impact factor: 3.857

9. Stimulated DNA synthesis in mouse epidermal cell cultures treated with 12-O-tetradecanoyl-phorbol-13-acetate.

Authors: S H Yuspa; T Ben; E Patterson; D Michael; K Elgjo; H Hennings
Journal: Cancer Res Date: 1976-11 Impact factor: 12.701

10. Keratin filaments of cultured human epidermal cells. Formation of intermolecular disulfide bonds during terminal differentiation.

Authors: T T Sun; H Green
Journal: J Biol Chem Date: 1978-03-25 Impact factor: 5.157

4 in total

1. Type I and type II keratins have evolved from lower eukaryotes to form the epidermal intermediate filaments in mammalian skin.

Authors: E Fuchs; D Marchuk
Journal: Proc Natl Acad Sci U S A Date: 1983-10 Impact factor: 11.205

2. Differences in keratin synthesis between normal epithelial cells and squamous cell carcinomas are mediated by vitamin A.

Authors: K H Kim; F Schwartz; E Fuchs
Journal: Proc Natl Acad Sci U S A Date: 1984-07 Impact factor: 11.205

3. Changes in quantities of high-mobility-group protein 1 in oviduct cellular fractions after oestrogen stimulation.

Authors: C T Teng; C S Teng
Journal: Biochem J Date: 1981-07-15 Impact factor: 3.857

4. Spontaneous immortalization of mouse liver sinusoidal endothelial cells.

Authors: Xiuhua Zhao; Qian Zhao; Zhen Luo; Yan Yu; Na Xiao; Xuan Sun; Lamei Cheng
Journal: Int J Mol Med Date: 2015-01-13 Impact factor: 4.101

4 in total