Literature DB >> 6153800

Method for preparing cultures of central neurons: cytochemical and immunochemical studies.

J Sotelo, C J Gibbs, D C Gajdusek, B H Toh, M Wurth.   

Abstract

We report a simplified method for culturing fetal central nervous system cells predominantly inducing neurons that grow, differentiate, and live in vitro for as long as 10 weeks. These central nervous system cells form a confluent cell culture in which about 80% of the cells are fully differentiated neurons producing interconnecting axons and dendrite processes and live upon a sparse underlying population of fibrillary and protoplasmic astrocytes, oligodendrocytes, and fibroblasts. Morphological and cytochemical characteristics of these cell types, based on immunofluorescent cell specific markers and silver staining of neurons, are presented.

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Year:  1980        PMID： 6153800      PMCID： PMC348333          DOI： 10.1073/pnas.77.1.653

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  11 in total

1.  Neurons from fetal rat brain in a new cell culture system: a multidisciplinary analysis.

Authors:  E W Godfrey; P G Nelson; B K Schrier; A C Breuer; B R Ransom
Journal:  Brain Res       Date:  1975-06-06       Impact factor: 3.252

2.  Glial fibrillary acidic protein in reaggregating and monolayer cultures of fetal mouse cerebral hemispheres.

Authors:  L P Kozak; D Dahl; A Bignami
Journal:  Brain Res       Date:  1978-07-21       Impact factor: 3.252

3.  The derivation and characterization of neuronal cell lines from rat and mouse brain.

Authors:  K Bulloch; W B Stallcup; M Cohn
Journal:  Brain Res       Date:  1977-10-21       Impact factor: 3.252

4.  Galactocerebroside is a specific cell-surface antigenic marker for oligodendrocytes in culture.

Authors:  M C Raff; R Mirsky; K L Fields; R P Lisak; S H Dorfman; D H Silberberg; N A Gregson; S Leibowitz; M C Kennedy
Journal:  Nature       Date:  1978-08-24       Impact factor: 49.962

5.  Dissociation, fractionation, and culture of embryonic brain cells.

Authors:  S Varon; C W Raiborn
Journal:  Brain Res       Date:  1969-01       Impact factor: 3.252

6.  Selection of differentiated neurons in embryonic mouse brain cultures using arabinofuranosylcytosine.

Authors:  R Dambergs; J Leah; C Kidson
Journal:  Exp Neurol       Date:  1978-04       Impact factor: 5.330

7.  Actin may be present on the lymphocyte surface.

Authors:  M J Owen; J Auger; B H Barber; A J Edwards; F S Walsh; M J Crumpton
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

8.  Actin co-caps with concanavalin A receptors.

Authors:  B H Toh; C C Hard
Journal:  Nature       Date:  1977-10-20       Impact factor: 49.962

9.  Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties.

Authors:  B R Ransom; E Neale; M Henkart; P N Bullock; P G Nelson
Journal:  J Neurophysiol       Date:  1977-09       Impact factor: 2.714

10.  Actin antibody: the specific visualization of actin filaments in non-muscle cells.

Authors:  E Lazarides; K Weber
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205
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  10 in total

1.  Monoclonal antibody analysis of keratin expression in the central nervous system.

Authors:  M C Franko; C J Gibbs; D A Rhoades; D C Gajdusek
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

2.  Influence of meningeal cells on the proliferation and maturation of rat neuroblasts in culture.

Authors:  C Gensburger; G Labourdette; M Sensenbrenner
Journal:  Exp Brain Res       Date:  1986       Impact factor: 1.972

3.  The 200- and 150-kDa neurofilament proteins react with IgG autoantibodies from chimpanzees with kuru or Creutzfeldt-Jakob disease; a 62-kDa neurofilament-associated protein reacts with sera from sheep with natural scrapie.

Authors:  B H Toh; C J Gibbs; D C Gajdusek; D D Tuthill; D Dahl
Journal:  Proc Natl Acad Sci U S A       Date:  1985-06       Impact factor: 11.205

4.  Heterogeneic autoantibody against neurofilament protein in the sera of animals with experimental kuru and Creutzfeldt-Jakob disease and natural scrapie infection.

Authors:  T Aoki; C J Gibbs; J Sotelo; D C Gajdusek
Journal:  Infect Immun       Date:  1982-10       Impact factor: 3.441

5.  The 200- and 150-kDa neurofilament proteins react with IgG autoantibodies from patients with kuru, Creutzfeldt-Jakob disease, and other neurologic diseases.

Authors:  B H Toh; C J Gibbs; D C Gajdusek; J Goudsmit; D Dahl
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

6.  Specific interactions between retrovirus Env and Gag proteins in rat neurons.

Authors:  K Weclewicz; M Ekström; K Kristensson; H Garoff
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

7.  Neurovirulence mutant of vesicular stomatitis virus with an altered target cell tropism in vivo.

Authors:  O T Preble; L E Costello; D D Huang; M A Barmada
Journal:  Infect Immun       Date:  1980-08       Impact factor: 3.441

8.  Immunoreactive insulin from mouse brain cells in culture and whole rat brain.

Authors:  N P Birch; D L Christie; A G Renwick
Journal:  Biochem J       Date:  1984-02-15       Impact factor: 3.857

9.  Characterization of a factor that promotes neurite outgrowth: evidence linking activity to a heparan sulfate proteoglycan.

Authors:  A D Lander; D K Fujii; D Gospodarowicz; L F Reichardt
Journal:  J Cell Biol       Date:  1982-09       Impact factor: 10.539

10.  Targeting of endoplasmic reticulum-associated proteins to axons and dendrites in rotavirus-infected neurons.

Authors:  K Weclewicz; L Svensson; K Kristensson
Journal:  Brain Res Bull       Date:  1998-07-01       Impact factor: 4.077
  10 in total

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