Literature DB >> 6149704

Simultaneous single-step purification of thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri.

M X Sliwkowski, M G Hartmanis.   

Abstract

Thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri were purified by ion-exchange chromatography to near homogeneity in a simultaneous, single-step procedure. The yield of both enzymes was greater than 80%. Thiolase was purified approximately 8-fold with sp act 115 units/mg, whereas 3-hydroxybutyryl-CoA dehydrogenase was purified 14-fold with sp act 292 units/mg. Isoelectric points of the enzymes are 7.7 for thiolase and 7.8 for 3-hydroxybutyryl-CoA dehydrogenase. Milligram quantities of each of these enzymes are readily obtained from this fatty acid-producing organism.

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Year:  1984        PMID： 6149704     DOI： 10.1016/0003-2697(84)90052-6

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Thiolase from Clostridium acetobutylicum ATCC 824 and Its Role in the Synthesis of Acids and Solvents.

Authors:  D P Wiesenborn; F B Rudolph; E T Papoutsakis
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

2.  Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase.

Authors:  Stefan R Kaschabek; Bernd Kuhn; Dagmar Müller; Eberhard Schmidt; Walter Reineke
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

3.  Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli.

Authors:  El-Hussiny Aboulnaga; Olaf Pinkenburg; Johannes Schiffels; Ahmed El-Refai; Wolfgang Buckel; Thorsten Selmer
Journal:  J Bacteriol       Date:  2013-06-14       Impact factor: 3.490

4.  Purification and characterization of the NADH-dependent (S)-specific 3-oxobutyryl-CoA reductase from Clostridium tyrobutyricum.

Authors:  M Bayer; H Günther; H Simon
Journal:  Arch Microbiol       Date:  1995-04       Impact factor: 2.552

5.  Purification and properties of 3-hydroxybutyryl-coenzyme A dehydrogenase from Clostridium beijerinckii ("Clostridium butylicum") NRRL B593.

Authors:  G D Colby; J S Chen
Journal:  Appl Environ Microbiol       Date:  1992-10       Impact factor: 4.792

6.  Production of medium-chain carboxylic acids by Megasphaera sp. MH with supplemental electron acceptors.

Authors:  Byoung Seung Jeon; Okkyoung Choi; Youngsoon Um; Byoung-In Sang
Journal:  Biotechnol Biofuels       Date:  2016-06-22       Impact factor: 6.040
  6 in total

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