Literature DB >> 6149651

Identification of the herpes simplex virus type 1 gene encoding the dUTPase.

V G Preston, F B Fisher.   

Abstract

The dUTPase gene of herpes simplex virus has been identified using a novel approach. The upstream regulatory sequences, or the promoter and upstream regulatory sequences, of the immediate-early gene Vmw175 (ICP4) were inserted in front of genes mapping in the region 0.69 to 0.70 map units of the virus genome to enhance transient gene expression in a transfection assay. One clone, containing a gene specifying a 1.5-kb mRNA, induced significant amounts of virus-specific dUTPase activity. The enzyme activity was abolished by insertion of a HindIII linker into the KpnI site within the coding sequences of this gene. The results show that the enzyme is virus coded and that 1.5-kb mRNA specifies the dUTPase.

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Year:  1984        PMID： 6149651     DOI： 10.1016/0042-6822(84)90147-8

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  29 in total

Review 1.  Recognition mechanisms in the synthesis of animal virus DNA.

Authors:  R T Hay; W C Russell
Journal:  Biochem J       Date:  1989-02-15       Impact factor: 3.857

2.  New genes from old: redeployment of dUTPase by herpesviruses.

Authors:  Andrew J Davison; Nigel D Stow
Journal:  J Virol       Date:  2005-10       Impact factor: 5.103

3.  Herpes simplex virus 1 protein kinase Us3 phosphorylates viral dUTPase and regulates its catalytic activity in infected cells.

Authors:  Akihisa Kato; Shumpei Tsuda; Zhuoming Liu; Hiroko Kozuka-Hata; Masaaki Oyama; Yasushi Kawaguchi
Journal:  J Virol       Date:  2013-10-30       Impact factor: 5.103

4.  Abundant non-canonical dUTP found in primary human macrophages drives its frequent incorporation by HIV-1 reverse transcriptase.

Authors:  Edward M Kennedy; Waaqo Daddacha; Rebecca Slater; Christina Gavegnano; Emilie Fromentin; Raymond F Schinazi; Baek Kim
Journal:  J Biol Chem       Date:  2011-03-31       Impact factor: 5.157

5.  Protein sequence comparisons show that the 'pseudoproteases' encoded by poxviruses and certain retroviruses belong to the deoxyuridine triphosphatase family.

Authors:  D J McGeoch
Journal:  Nucleic Acids Res       Date:  1990-07-25       Impact factor: 16.971

6.  Phosphorylation of herpes simplex virus 1 dUTPase regulates viral virulence and genome integrity by compensating for low cellular dUTPase activity in the central nervous system.

Authors:  Akihisa Kato; Jun Arii; Yoshio Koyanagi; Yasushi Kawaguchi
Journal:  J Virol       Date:  2014-10-15       Impact factor: 5.103

7.  Isolation and characterization of herpes simplex virus type 1 host range mutants defective in viral DNA synthesis.

Authors:  E P Carmichael; M J Kosovsky; S K Weller
Journal:  J Virol       Date:  1988-01       Impact factor: 5.103

8.  High-molecular-weight protein (pUL48) of human cytomegalovirus is a competent deubiquitinating protease: mutant viruses altered in its active-site cysteine or histidine are viable.

Authors:  Jianlei Wang; Amy N Loveland; Lisa M Kattenhorn; Hidde L Ploegh; Wade Gibson
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

9.  Phosphorylation of a herpes simplex virus 1 dUTPase by a viral protein kinase, Us3, dictates viral pathogenicity in the central nervous system but not at the periphery.

Authors:  Akihisa Kato; Keiko Shindo; Yuhei Maruzuru; Yasushi Kawaguchi
Journal:  J Virol       Date:  2013-12-18       Impact factor: 5.103

10.  Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice.

Authors:  Frank S De Silva; Bernard Moss
Journal:  Virol J       Date:  2008-12-02       Impact factor: 4.099
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