Literature DB >> 6147283

Partial purification of rat liver cytoplasmic acetyl-CoA synthetase; characterization of some properties.

E Imesch, S Rous.   

Abstract

Rat liver cytoplasmic acetyl-CoA synthetase was partially purified (purification factor = 23, yield = 30%). The apparent Kms for acetate, coenzyme A, ATP and MgCl2 were determined and found to be 52.5 microM, 50.5 microM, 570 microM and 1.5 mM, respectively. The partially-purified enzyme showed a low affinity for short-chain carbon substrates other than acetate. The properties of the partially-purified enzyme were compared with those of enzymes from other sources.

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Year:  1984        PMID： 6147283     DOI： 10.1016/0020-711x(84)90146-0

Source DB:  PubMed          Journal:  Int J Biochem        ISSN： 0020-711X


  6 in total

1.  Nuclear-cytoplasmic localization of acetyl coenzyme a synthetase-1 in the rat brain.

Authors:  Prasanth S Ariyannur; John R Moffett; Chikkathur N Madhavarao; Peethambaran Arun; Nisha Vishnu; David M Jacobowitz; William C Hallows; John M Denu; Aryan M A Namboodiri
Journal:  J Comp Neurol       Date:  2010-08-01       Impact factor: 3.215

2.  Peroxisomal oxidation of erucic acid suppresses mitochondrial fatty acid oxidation by stimulating malonyl-CoA formation in the rat liver.

Authors:  Xiaocui Chen; Lin Shang; Senwen Deng; Ping Li; Kai Chen; Ting Gao; Xiao Zhang; Zhilan Chen; Jia Zeng
Journal:  J Biol Chem       Date:  2020-06-03       Impact factor: 5.157

3.  Purification and characterization of two reversible and ADP-dependent acetyl coenzyme A synthetases from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  X Mai; M W Adams
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

4.  Purification of Pseudomonas putida acyl coenzyme A ligase active with a range of aliphatic and aromatic substrates.

Authors:  M Fernández-Valverde; A Reglero; H Martinez-Blanco; J M Luengo
Journal:  Appl Environ Microbiol       Date:  1993-04       Impact factor: 4.792

5.  The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds.

Authors:  J Ke; R H Behal; S L Back; B J Nikolau; E S Wurtele; D J Oliver
Journal:  Plant Physiol       Date:  2000-06       Impact factor: 8.005

Review 6.  N-Acetylaspartate reductions in brain injury: impact on post-injury neuroenergetics, lipid synthesis, and protein acetylation.

Authors:  John R Moffett; Peethambaran Arun; Prasanth S Ariyannur; Aryan M A Namboodiri
Journal:  Front Neuroenergetics       Date:  2013-12-26
  6 in total

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